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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
2001-8-29
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pubmed:abstractText |
The ability of Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus administered in yogurt to survive the passage through the upper gastrointestinal tract was investigated with Göttingen minipigs that were fitted with ileum T-cannulas. After ingestion of yogurt containing viable microorganisms, ileostomy samples were collected nearly every hour beginning 3 h after food uptake. Living L. delbrueckii subsp. bulgaricus and S. thermophilus were detected in the magnitude of 10(6) to 10(7) per gram of intestinal contents (wet weight) in all animals under investigation. A calculation of the minimum amount of surviving bacteria that had been administered is presented. Total DNA extracted from ileostomy samples was subjected to PCR, which was species specific for L. delbrueckii and S. thermophilus and subspecies specific for L. delbrueckii subsp. bulgaricus. All three bacterial groups could be detected by PCR after yogurt uptake but not after uptake of a semisynthetic diet. One pig apparently had developed an endogenous L. delbrueckii flora. When heat-treated yogurt was administered, L. delbrueckii was detected in all animals. S. thermophilus or L. delbrueckii subsp. bulgaricus was not detected, indicating that heat-inactivated cells and their DNAs had already been digested and their own L. delbrueckii flora had been stimulated for growth.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11526016-10488843,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11526016-10532384,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11526016-10543799,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11526016-10543808,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11526016-10930078,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11526016-1728822,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11526016-2497633,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11526016-3106442,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11526016-7618890,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11526016-8398215,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11526016-8439146,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11526016-8939025,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11526016-9040550,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11526016-9201571
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0099-2240
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
67
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4137-43
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:11526016-Animals,
pubmed-meshheading:11526016-Colony Count, Microbial,
pubmed-meshheading:11526016-DNA, Bacterial,
pubmed-meshheading:11526016-Diet,
pubmed-meshheading:11526016-Gastrointestinal Contents,
pubmed-meshheading:11526016-Ileostomy,
pubmed-meshheading:11526016-Ileum,
pubmed-meshheading:11526016-Lactobacillus,
pubmed-meshheading:11526016-Models, Animal,
pubmed-meshheading:11526016-Polymerase Chain Reaction,
pubmed-meshheading:11526016-Probiotics,
pubmed-meshheading:11526016-Streptococcus,
pubmed-meshheading:11526016-Swine,
pubmed-meshheading:11526016-Swine, Miniature,
pubmed-meshheading:11526016-Yogurt
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pubmed:year |
2001
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pubmed:articleTitle |
Survival of Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus in the terminal ileum of fistulated Göttingen minipigs.
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pubmed:affiliation |
Institute for Microbiology, Federal Dairy Research Center, D-24103 Kiel, Germany. lick@bafm.de
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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