Source:http://linkedlifedata.com/resource/pubmed/id/11523787
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
2001-8-28
|
| pubmed:abstractText |
By screening for Arabidopsis genes activated by ionising radiation (IR)-induced DNA damage, we have isolated a cDNA hybridising with a 3.2-kb mRNA that accumulates rapidly and strongly in irradiated cell suspensions or whole plants. The cDNA codes for a 110-kDa protein that is highly homologous to the 116-kDa vertebrate poly(ADP-ribose) polymerase (PARP-1). It is recognised by a human anti-PARP-1 antibody, binds efficiently to DNA strand interruptions in vitro, and catalyses DNA damage-dependent (ADP-ribose) polymer synthesis. We have named this protein AtPARP-1. We have also extended our observations to the Arabidopsis app (AtPARP-2) gene, demonstrating for the first time that IR-induced DNA strand interruptions induce rapid and massive accumulation of AtPARP-1 and AtPARP-2 transcripts, whereas dehydration and cadmium preferentially induce the accumulation of AtPARP-2 transcripts. The IR-induced PARP gene expression seen in Arabidopsis is in striking contrast to the post-translational activation of the PARP-1 protein that is associated with genotoxic stress in animal cells. AtPARP-1 transcripts accumulate in all plant organs after exposure to ionising radiation, but this is followed by an increase in AtPARP-1 protein levels only in tissues that contain large amounts of actively dividing cells. This cell-type specific accumulation of AtPARP-1 protein in response to DNA damage is compatible with a role for the AtPARP-1 protein in the maintenance of DNA integrity during replication, similar to the role of "guardian of the genome" attributed to its animal counterpart.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cobalt Radioisotopes,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Poly(ADP-ribose) Polymerases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Plant
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
1617-4615
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
265
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
954-63
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:11523787-Amino Acid Sequence,
pubmed-meshheading:11523787-Animals,
pubmed-meshheading:11523787-Arabidopsis,
pubmed-meshheading:11523787-Catalytic Domain,
pubmed-meshheading:11523787-Cobalt Radioisotopes,
pubmed-meshheading:11523787-Conserved Sequence,
pubmed-meshheading:11523787-Gamma Rays,
pubmed-meshheading:11523787-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:11523787-Gene Expression Regulation, Plant,
pubmed-meshheading:11523787-Humans,
pubmed-meshheading:11523787-Isoenzymes,
pubmed-meshheading:11523787-Molecular Sequence Data,
pubmed-meshheading:11523787-Open Reading Frames,
pubmed-meshheading:11523787-Poly(ADP-ribose) Polymerases,
pubmed-meshheading:11523787-RNA, Messenger,
pubmed-meshheading:11523787-RNA, Plant,
pubmed-meshheading:11523787-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:11523787-Sequence Alignment,
pubmed-meshheading:11523787-Sequence Homology, Amino Acid,
pubmed-meshheading:11523787-Transcription, Genetic,
pubmed-meshheading:11523787-Vertebrates,
pubmed-meshheading:11523787-Zinc Fingers
|
| pubmed:year |
2001
|
| pubmed:articleTitle |
Ionising radiation induces the expression of PARP-1 and PARP-2 genes in Arabidopsis.
|
| pubmed:affiliation |
CEA/Cadarache, DSV-DEVM-Laboratoire de Radiobiologie Végétale, Saint Paul-lez-Durance, France.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|