Linked Life Data

11522255

Source:http://linkedlifedata.com/resource/pubmed/id/11522255

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2001-8-27
pubmed:abstractText
The plastoquinone pool during dark adaptation is reduced by endogenous reductants and oxidized at the expense of molecular oxygen. We report here on the redox state of plastoquinone in darkness, using as an indicator the chlorophyll fluorescence kinetics of whole cells of a Chlamydomonas reinhardtii mutant strain lacking the cytochrome b(6)f complex. When algae were equilibrated with a mixture of air and argon at 1.45% air, plastoquinol oxidation was inhibited whereas mitochondrial respiration was not. Consequently, mitochondrial oxidases cannot be responsible for the oxygen consumption linked to plastoquinol oxidation. Plastoquinol oxidation in darkness turned out to be sensitive to n-propyl gallate (PG) and insensitive to salicylhydroxamic acid (SHAM), whereas mitochondrial respiration was sensitive to SHAM and PG. Thus, both PG treatment and partial anaerobiosis allow to draw a distinction between an inhibition of plastoquinol oxidation and an inhibition of mitochondrial respiration, indicating the presence of a plastoquinol:oxygen oxidoreductase. The possible identification of this oxidase with an oxidase involved in carotenoid biosynthesis is discussed in view of various experimental data.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
17
pubmed:volume
1506
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
133-42
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
Chlororespiration and the process of carotenoid biosynthesis.
pubmed:affiliation
Institut de Biologie Physico-Chimique, Centre National de la Recherche Scientifique, UPR 1261, 13, rue Pierre et Marie Curie, 75005, Paris, France. pierre.bennoun@ibpc.fr
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't