Linked Life Data

11517607

Source:http://linkedlifedata.com/resource/pubmed/id/11517607

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2001-8-23
pubmed:abstractText
To investigate the effects of nutrition condition on plasmid DNA production, Escherichia coli JM109 containing plasmid pcDNA3S-HBA was grown in different culture media. Results show that carbon source, nitrogen source affected plasmid DNA yield significantly. Glucose and peptone was selected, since they were preferable to other investigated carbon source and nitrogen source. In M9P medium, the appropriate concentration of(NH4)2SO4 was important for plasmid DNA production, Gly, Asp, Gln were formulated into the M9G medium because they served as the nitrogen donors for nucleotides synthesis. With addition of 1.2 g/L Asp, 1.0 g/L Gln and 0.4 g/L Gly into M9G medium, plasmid DNA yield was 25 mg/L after 20 h culture. Foreign nucleotides distinctively affected plasmid DNA production. With addition of 0.4 g/L nucleosides mixture of thymidine and cytidine(moles fraction of thymidine:cytidine = 1:1) into M9P medium, plasmid DNA yield was 35 mg/L.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1000-3061
pubmed:author
pubmed:issnType
Print
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
310-3
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
[Effects of nutrition condition on pcDNA3-HBS plasmid DNA production].
pubmed:affiliation
Animal Science Research Institute, Wuxi University of Light Industry, Wuxi 214036, China.
pubmed:publicationType
Journal Article, English Abstract, Research Support, Non-U.S. Gov't