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rdf:type |
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lifeskim:mentions |
umls-concept:C0019682,
umls-concept:C0019699,
umls-concept:C0024485,
umls-concept:C0039194,
umls-concept:C0205307,
umls-concept:C0871261,
umls-concept:C1450054,
umls-concept:C1515655,
umls-concept:C1704632,
umls-concept:C1706817,
umls-concept:C2346689,
umls-concept:C2911692
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pubmed:issue |
1-2
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pubmed:dateCreated |
2001-8-22
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pubmed:abstractText |
The present study analyzed the feasibility of using magnetic resonance imaging (MRI) to monitor T-cell homing in vivo after loading T cells with superparamagnetic iron oxide (CLIO) nanoparticles derivatized with a peptide sequence from the transactivator protein (Tat) of HIV-1. T cells were isolated from C57BL/6 (B6) mice and loaded with 0, 400, 800, 1600, or 8000 ng/ml of FITC conjugated CLIO-Tat (FITC-CLIO-Tat). There was a dose-dependent uptake of FITC-CLIO-Tat by T cells. Stimulation of FITC-CLIO-Tat loaded T cells with anti-CD3 (0.1 microg/ml) plus IL-2 (5 ng/ml) elicited normal activation and activation-induced cell death (AICD) responses, and normal upregulation of CD69, ICAM-1 (CD54), L-selectin (CD62L), and Fas. The FITC-CLIO-Tat loaded T cells (3 x 10(7)) were transferred intravenously (i.v.) into B6 mice and the in vivo MRI of mice was acquired using a spin-echo pulse sequence at 4.7 T with a Bruker Biospec system. Homing of T cells into the spleen was observed by a decrease in MRI signal intensity within 1 h after the transfer, which remained decreased for 2-24 h after transfer. These homing data were confirmed by FACS analysis and biodistribution analysis using 125I-CLIO-Tat. Thus, T cells can be efficiently loaded with FITC-CLIO-Tat without interfering with their normal activation and AICD, or homing to the spleen, and the biodistribution of FITC-CLIO-Tat loaded T cells can be monitored in vivo over time by MRI.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD95,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Thy-1,
http://linkedlifedata.com/resource/pubmed/chemical/Ferric Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescein-5-isothiocyanate,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, tat,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Adhesion Molecule-1,
http://linkedlifedata.com/resource/pubmed/chemical/L-Selectin,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/ferric oxide
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0022-1759
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pubmed:author |
pubmed-author:ChuW JWJ,
pubmed-author:DoddC HCH,
pubmed-author:ForderJJ,
pubmed-author:HsuH CHC,
pubmed-author:JosephsonLL,
pubmed-author:MountzJ DJD,
pubmed-author:MountzJ DJDJr,
pubmed-author:MountzJ MJM,
pubmed-author:WeisslederRR,
pubmed-author:YangPP,
pubmed-author:ZhangH GHG,
pubmed-author:ZinkHH
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
256
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
89-105
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:11516758-Animals,
pubmed-meshheading:11516758-Antigens, CD95,
pubmed-meshheading:11516758-Antigens, Thy-1,
pubmed-meshheading:11516758-Apoptosis,
pubmed-meshheading:11516758-Cell Movement,
pubmed-meshheading:11516758-Cells, Cultured,
pubmed-meshheading:11516758-Female,
pubmed-meshheading:11516758-Ferric Compounds,
pubmed-meshheading:11516758-Fluorescein-5-isothiocyanate,
pubmed-meshheading:11516758-Gene Products, tat,
pubmed-meshheading:11516758-Intercellular Adhesion Molecule-1,
pubmed-meshheading:11516758-L-Selectin,
pubmed-meshheading:11516758-Lymphocyte Activation,
pubmed-meshheading:11516758-Magnetic Resonance Imaging,
pubmed-meshheading:11516758-Mice,
pubmed-meshheading:11516758-Mice, Inbred C57BL,
pubmed-meshheading:11516758-Peptides,
pubmed-meshheading:11516758-Spleen,
pubmed-meshheading:11516758-T-Lymphocytes
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pubmed:year |
2001
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pubmed:articleTitle |
Normal T-cell response and in vivo magnetic resonance imaging of T cells loaded with HIV transactivator-peptide-derived superparamagnetic nanoparticles.
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pubmed:affiliation |
Division of Clinical Immunology and Rheumatology, Department of Medicine, The University of Alabama at Birmingham, 35294, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't,
Evaluation Studies
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