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pubmed-article:11516335pubmed:abstractTextThe development and activation of CD4+ helper T cell (Th) subsets with distinct patterns of unbalanced production of cytokines play an important part in infectious, allergic and autoimmune diseases. Human neonatal cord blood CD4+ Th cells can be polarized into type 1 or type 2-like effector cells in vitro by culturing them in the presence of interleukin (IL)-12 or IL-4, respectively. We have exploited this experimental system to identify marker genes that are differentially expressed by polarized Th1 and Th2 cells. An oligonucleotide microarray specifically designed to screen for inflammation-related candidate genes was used and the differential expression was further validated with a quantitative real-time RT-PCR method.lld:pubmed
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pubmed-article:11516335pubmed:authorpubmed-author:HellerRRlld:pubmed
pubmed-article:11516335pubmed:authorpubmed-author:HashizumeHHlld:pubmed
pubmed-article:11516335pubmed:authorpubmed-author:LahesmaaRRlld:pubmed
pubmed-article:11516335pubmed:authorpubmed-author:ZhouHHlld:pubmed
pubmed-article:11516335pubmed:authorpubmed-author:ChoySSlld:pubmed
pubmed-article:11516335pubmed:authorpubmed-author:HamalainenHHlld:pubmed
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pubmed-article:11516335pubmed:paginationRESEARCH0022lld:pubmed
pubmed-article:11516335pubmed:dateRevised2009-11-18lld:pubmed
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pubmed-article:11516335pubmed:year2001lld:pubmed
pubmed-article:11516335pubmed:articleTitleDistinct gene expression profiles of human type 1 and type 2 T helper cells.lld:pubmed
pubmed-article:11516335pubmed:affiliationTurku Centre for Biotechnology, University of Turku and Abo Akademi University, FIN-20521 Turku, Finland.lld:pubmed
pubmed-article:11516335pubmed:publicationTypeJournal Articlelld:pubmed
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