Source:http://linkedlifedata.com/resource/pubmed/id/11516127
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
2001-8-22
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pubmed:abstractText |
The objective of this study was to monitor the changes in semen characteristics in vasectomized rams and to determine if infertility was present 14 days after vasectomy. Experiments were performed using five cross-breed rams, aged between 18 and 30 months. Semen was collected weekly by artificial vagina from 2 months before to 5 months after vasectomy. After sexual rest for 10 days, vasectomy was performed by the cranial midscrotal approach. In all ejaculates the volume, concentration, total sperm number, motility and morphology (normal spermatozoa, loose heads) were determined and sperm viability (SYBR-14/PI) was evaluated in all semen samples collected after vasectomy. In the first ejaculate obtained 14 days post vasectomy all rams showed a significant (P < 0.05) drop in mean volume (from 1.2 to 0.5 mL), total sperm count (from 5176.8 to 51.1 x 10(6)) and morphologically normal sperm (from 84.1 to 15.7%), when compared to the last prevasectomy collection. We could also demonstrate a positive correlation (r = 0.89) between the individual cumulative total number of spermatozoa after vasectomy and the scrotal circumference measured before vasectomy. Sperm motility and viability could never be demonstrated after vasectomy and normal spermatozoa continuously decreased concomitant with an increase in loose heads. On post mortem examination 5 months after surgery, spermatocele formation and multiple sperm granulomas were present in all five rams. Our results show that in the first ejaculate collected by artificial vagina 14 days after vasectomy, no motile and viable spermatozoa could be detected. Despite weekly collections during a 5-month period after sterilization, azoospermia could never be achieved.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Coloring Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Organic Chemicals,
http://linkedlifedata.com/resource/pubmed/chemical/Propidium,
http://linkedlifedata.com/resource/pubmed/chemical/SYBR-14
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0093-691X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
56
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
485-91
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pubmed:dateRevised |
2005-11-17
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pubmed:meshHeading |
pubmed-meshheading:11516127-Animals,
pubmed-meshheading:11516127-Coloring Agents,
pubmed-meshheading:11516127-Fluorescent Dyes,
pubmed-meshheading:11516127-Infertility, Male,
pubmed-meshheading:11516127-Male,
pubmed-meshheading:11516127-Microscopy, Fluorescence,
pubmed-meshheading:11516127-Organic Chemicals,
pubmed-meshheading:11516127-Propidium,
pubmed-meshheading:11516127-Scrotum,
pubmed-meshheading:11516127-Semen,
pubmed-meshheading:11516127-Sheep,
pubmed-meshheading:11516127-Sperm Count,
pubmed-meshheading:11516127-Sperm Motility,
pubmed-meshheading:11516127-Spermatozoa,
pubmed-meshheading:11516127-Vasectomy
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pubmed:year |
2001
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pubmed:articleTitle |
Semen characteristics after vasectomy in the ram.
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pubmed:affiliation |
Department of Reproduction, University of Zürich, Switzerland. janett@vetangy.unizh.ch
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pubmed:publicationType |
Journal Article
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