Source:http://linkedlifedata.com/resource/pubmed/id/11514536
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
42
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| pubmed:dateCreated |
2001-10-15
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| pubmed:abstractText |
Both cell-specific and ubiquitous transcription factors in fibroblasts have been identified as critical for expression of the Col1a1 gene, which encodes the alpha1 chain of type I collagen. Here, we report that Yin Yang 1 (YY1) binds to the Col1a1 promoter immediately upstream of the TATA box, and we examine the functional implications of YY1 binding for regulation of Col1a1 gene expression in BALBc/3T3 fibroblasts. The Col1a1 promoter region spanning base pairs (bp) -56 to -9 bound purified recombinant YY1 and the corresponding binding activity in nuclear extracts was supershifted using a YY1-specific antibody. Mutation of the TATA box to TgTA enhanced YY1 complex formation. Mutation analysis revealed two YY1 core binding sites at -40/-37 bp (YY1A) and, on the reverse strand, at -32/-29 bp (YY1B) immediately adjacent to the TATA box. In transfections using Col1a1-luciferase constructs, mutation of YY1A decreased activity completely (wild-type p350 (p350wt), -222/+113 bp) or partially (p130wt, -84 bp/+13 bp), whereas mutation of YY1B blocked the expression of both promoter constructs. Cotransfection with pCMV-YY1 increased p350wt and p130wt activities by as much as 10-fold, whereas antisense YY1 decreased constitutive expression and blocked the increased activity due to pCMV-YY1 overexpression. The mTgTA constructs were devoid of activity, arguing for a requirement for cognate binding of the TATA box-binding protein (TBP). Electrophoretic mobility shift assays performed under conditions permitting TBP binding showed that recombinant TBP/TFIID and YY1 could bind to the -56/-9 bp fragment and that YY1B was the preferred site for YY1 binding. Our results indicate that YY1 binds to the Col1a1 proximal promoter and functions as a positive regulator of constitutive activity in fibroblasts. Although YY1 is not sufficient for transcriptional initiation, it is a required component of the transcription machinery in this promoter.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Collagen,
http://linkedlifedata.com/resource/pubmed/chemical/Collagen Type I,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Erythroid-Specific DNA-Binding...,
http://linkedlifedata.com/resource/pubmed/chemical/Luciferases,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides, Antisense,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor TFIID,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors, TFII,
http://linkedlifedata.com/resource/pubmed/chemical/YY1 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Yy1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/collagen type I, alpha 1 chain
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| pubmed:status |
MEDLINE
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| pubmed:month |
Oct
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| pubmed:issn |
0021-9258
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
19
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| pubmed:volume |
276
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
38665-72
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:11514536-3T3 Cells,
pubmed-meshheading:11514536-Animals,
pubmed-meshheading:11514536-Base Sequence,
pubmed-meshheading:11514536-Binding Sites,
pubmed-meshheading:11514536-Cell Nucleus,
pubmed-meshheading:11514536-Collagen,
pubmed-meshheading:11514536-Collagen Type I,
pubmed-meshheading:11514536-DNA Mutational Analysis,
pubmed-meshheading:11514536-DNA-Binding Proteins,
pubmed-meshheading:11514536-Erythroid-Specific DNA-Binding Factors,
pubmed-meshheading:11514536-Gene Expression Regulation,
pubmed-meshheading:11514536-Luciferases,
pubmed-meshheading:11514536-Mice,
pubmed-meshheading:11514536-Molecular Sequence Data,
pubmed-meshheading:11514536-Mutation,
pubmed-meshheading:11514536-Oligonucleotides, Antisense,
pubmed-meshheading:11514536-Promoter Regions, Genetic,
pubmed-meshheading:11514536-Protein Binding,
pubmed-meshheading:11514536-Transcription, Genetic,
pubmed-meshheading:11514536-Transcription Factor TFIID,
pubmed-meshheading:11514536-Transcription Factors,
pubmed-meshheading:11514536-Transcription Factors, TFII,
pubmed-meshheading:11514536-Transfection,
pubmed-meshheading:11514536-YY1 Transcription Factor
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| pubmed:year |
2001
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| pubmed:articleTitle |
YY1 is a positive regulator of transcription of the Col1a1 gene.
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| pubmed:affiliation |
Beth Israel Deaconess Medical Center, Division of Rheumatology, and New England Baptist Bone & Joint Institute, Harvard Institutes of Medicine, Boston, Massachusetts 02115, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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