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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2001-8-21
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pubmed:abstractText |
Endothelial nitric oxide synthase (eNOS) is regulated both by caveolin-1 and 17beta-estradiol (E(2)). Temporal relationships between effects of estrogen on caveolin-1 and nitric oxide (NO) are not known. Therefore, this study was designed to determine whether estrogen regulates caveolin-1 and, if so, whether such regulation corresponds to changes in nitrite/nitrate (NO(x)) production. Bovine aortic endothelial cells (BAECs) were cultured in the absence and presence of 17beta-estradiol or 17alpha-estradiol (10(-8) and 10(-10) M) for 12, 24, and 48 h. eNOS protein expression and NO(x) production increased significantly after 24 h but not after 12-h treatment with 17beta- and not 17alpha-estradiol. Both mRNA and protein for caveolin-1 were increased significantly only after 48-h treatment with E(2), but eNOS protein and NO(x) production were decreased compared with cells treated for 24 h. These increases in caveolin-1 were inhibited by the estrogen receptor antagonist ICI-182,780 (10(-6) M). Results of this study suggest that E(2) stimulates caveolin-1 transcription and translation through estrogen receptor-mediated mechanisms. The results further suggest that estrogen may indirectly regulate NO(x) through caveolin-1 expression, which inhibits eNOS catalytic activity.
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pubmed:grant |
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Caveolin 1,
http://linkedlifedata.com/resource/pubmed/chemical/Caveolins,
http://linkedlifedata.com/resource/pubmed/chemical/Estradiol,
http://linkedlifedata.com/resource/pubmed/chemical/Estrogen Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Nitrates,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase Type III,
http://linkedlifedata.com/resource/pubmed/chemical/Nitrites,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/fulvestrant
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0363-6135
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
281
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
H1327-33
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:11514304-Animals,
pubmed-meshheading:11514304-Aorta,
pubmed-meshheading:11514304-Cattle,
pubmed-meshheading:11514304-Caveolin 1,
pubmed-meshheading:11514304-Caveolins,
pubmed-meshheading:11514304-Cells, Cultured,
pubmed-meshheading:11514304-Endothelium, Vascular,
pubmed-meshheading:11514304-Enzyme Activation,
pubmed-meshheading:11514304-Estradiol,
pubmed-meshheading:11514304-Estrogen Antagonists,
pubmed-meshheading:11514304-Female,
pubmed-meshheading:11514304-Gene Expression,
pubmed-meshheading:11514304-Male,
pubmed-meshheading:11514304-Nitrates,
pubmed-meshheading:11514304-Nitric Oxide Synthase,
pubmed-meshheading:11514304-Nitric Oxide Synthase Type III,
pubmed-meshheading:11514304-Nitrites,
pubmed-meshheading:11514304-RNA, Messenger,
pubmed-meshheading:11514304-Stereoisomerism,
pubmed-meshheading:11514304-Time Factors
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pubmed:year |
2001
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pubmed:articleTitle |
Temporal effects of 17beta-estradiol on caveolin-1 mRNA and protein in bovine aortic endothelial cells.
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pubmed:affiliation |
Department of Geriatrics, Nagoya University School of Medicine, Nagoya 466-8550, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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