Linked Life Data

11512494

Source:http://linkedlifedata.com/resource/pubmed/id/11512494

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-3
pubmed:dateCreated
2001-8-21
pubmed:abstractText
Rat islet cells in culture are able to form tridimensional aggregates with an architecture and functional activity similar to native islets: pseudoislets. Pseudoislets represent an alternative source for islet transplantation, because their transplant results in a long term allograft acceptance without immunosuppression of the host. Use of pseudoislets has been limited by their reduced yield and by poor reaggregation mass. Since culture conditions have been reported to affect reaggregation, the aim of this study was to evaluate the effects of different concentrations of two sera (Fetal Bovine Serum [FBS] and Rat Serum [RS]) on reaggregation and insulin gene expression in pseudoislets. Islets were isolated from male Lewis rat by means of histopaque gradient centrifugation. The day after islets were disrupted into single cells and cultured in RPMI 1640 5.6 mM glucose with 2%, 5% and 10% solutions of both FBS and RS. Cells spontaneously reaggregated to form pseudoislets. After seven days of culture, pseudoislets were counted and analysed for insulin secretion and insulin gene expression using RT-PCR. Rat serum increased the number of aggregates and their diameters. Insulin gene expression of pseudoislets cultured with RS showed a ten fold increase in comparison to those cultured with FBS. These data show that the culture medium supplemented with RS improves total reaggregate volume and increases insulin gene expression. With the perspective of pseudoislets' use in transplantation RS is better indicated than FBS for the production of rat pseudoislets.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0037-8771
pubmed:author
pubmed:issnType
Print
pubmed:volume
77
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
13-9
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:articleTitle
Rat serum improves rat pseudoislet formation and insulin gene expression.
pubmed:affiliation
DI.M.I., Dipartimento di Medicina Interna, Università di Genova.
pubmed:publicationType
Journal Article