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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
2001-8-20
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pubmed:databankReference |
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pubmed:abstractText |
We have isolated a murine cDNA coding for a beta1,3-N-acetylglucosaminyltransferase enzyme ( beta3GnT). This enzyme is similar in sequence to Drosophila melanogaster Brainiac and to the murine and human beta1,3-galactosyltransferase family of proteins. The mouse beta 3GnT protein is 397 amino acids in length and contains 7 cysteine residues that are conserved in the human orthologue. beta 3GnT is a type II membrane protein localized to the Golgi apparatus. Enzyme assays with recombinant mouse beta 3GnT reveal that it has a preference for acceptors with Gal(beta1-4)Glc(NAc) at the non-reducing termini. Proton NMR analysis of product showed incorporation of GlcNAc in beta1,3 linkage to the terminal Gal of Gal(beta1-4)Glc(beta1-O-benzyl). Northern blot analysis revealed the presence of a single 3.0[emsp4 ]kb transcript in all adult mouse and human organs tested, with highest levels in the kidney, liver, heart and placenta. The beta 3GnT gene is also expressed in a number of tumor cell lines. The human orthologue of beta 3GnT is located on chromosome 2pl5.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0282-0080
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
17
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
867-75
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11511811-Amino Acid Sequence,
pubmed-meshheading:11511811-Animals,
pubmed-meshheading:11511811-Base Sequence,
pubmed-meshheading:11511811-COS Cells,
pubmed-meshheading:11511811-Chromosome Mapping,
pubmed-meshheading:11511811-Chromosomes, Human, Pair 2,
pubmed-meshheading:11511811-Cloning, Molecular,
pubmed-meshheading:11511811-Drosophila Proteins,
pubmed-meshheading:11511811-Drosophila melanogaster,
pubmed-meshheading:11511811-Gene Expression Profiling,
pubmed-meshheading:11511811-Golgi Apparatus,
pubmed-meshheading:11511811-Humans,
pubmed-meshheading:11511811-Magnetic Resonance Spectroscopy,
pubmed-meshheading:11511811-Membrane Proteins,
pubmed-meshheading:11511811-Mice,
pubmed-meshheading:11511811-Molecular Sequence Data,
pubmed-meshheading:11511811-N-Acetylglucosaminyltransferases,
pubmed-meshheading:11511811-RNA, Messenger,
pubmed-meshheading:11511811-Recombinant Proteins,
pubmed-meshheading:11511811-Sequence Homology, Amino Acid,
pubmed-meshheading:11511811-Substrate Specificity
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pubmed:year |
2000
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pubmed:articleTitle |
Molecular cloning and expression analysis of a mouse UDP-GlcNAc:Gal(beta1-4)Glc(NAc)-R beta1,3-N-acetylglucosaminyltransferase homologous to Drosophila melanogaster Brainiac and the beta1,3-galactosyltransferase family.
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pubmed:affiliation |
The Programs in Developmental Biology, The Hospital for Sick Children, 555 University Avenue, Toronto, ON M5G 1X8, Canada. segan@sickkids.on.ca
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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