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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2001-8-20
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pubmed:databankReference |
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pubmed:abstractText |
Using X-ray crystallography, we have directly observed the path of mRNA in the 70S ribosome in Fourier difference maps at 7 A resolution. About 30 nucleotides of the mRNA are wrapped in a groove that encircles the neck of the 30S subunit. The Shine-Dalgarno helix is bound in a large cleft between the head and the back of the platform. At the interface, only about eight nucleotides (-1 to +7), centered on the junction between the A and P codons, are exposed, and bond almost exclusively to 16S rRNA. The mRNA enters the ribosome around position +13 to +15, the location of downstream pseudoknots that stimulate -1 translational frame shifting.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Codon,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Subunits,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Ribosomal, 16S,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/gp32 protein, Enterobacteria phage...
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0092-8674
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
27
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pubmed:volume |
106
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
233-41
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:11511350-Bacteriophage T4,
pubmed-meshheading:11511350-Base Pairing,
pubmed-meshheading:11511350-Base Sequence,
pubmed-meshheading:11511350-Binding Sites,
pubmed-meshheading:11511350-Codon,
pubmed-meshheading:11511350-Crystallography, X-Ray,
pubmed-meshheading:11511350-DNA-Binding Proteins,
pubmed-meshheading:11511350-Escherichia coli,
pubmed-meshheading:11511350-Fourier Analysis,
pubmed-meshheading:11511350-Frameshifting, Ribosomal,
pubmed-meshheading:11511350-Models, Molecular,
pubmed-meshheading:11511350-Molecular Sequence Data,
pubmed-meshheading:11511350-Nucleic Acid Conformation,
pubmed-meshheading:11511350-Protein Binding,
pubmed-meshheading:11511350-Protein Conformation,
pubmed-meshheading:11511350-Protein Subunits,
pubmed-meshheading:11511350-RNA, Messenger,
pubmed-meshheading:11511350-RNA, Ribosomal, 16S,
pubmed-meshheading:11511350-Ribosomes,
pubmed-meshheading:11511350-Thermus thermophilus,
pubmed-meshheading:11511350-Viral Proteins
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pubmed:year |
2001
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pubmed:articleTitle |
The path of messenger RNA through the ribosome.
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pubmed:affiliation |
Center for Molecular Biology of RNA, Sinsheimer Laboratories, University of California-Santa Cruz, Santa Cruz, CA 95064, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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