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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2001-8-20
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pubmed:abstractText |
Plasma membrane wounds are repaired by a mechanism involving Ca(2+)-regulated exocytosis. Elevation in intracellular [Ca(2+)] triggers fusion of lysosomes with the plasma membrane, a process regulated by the lysosomal synaptotagmin isoform Syt VII. Here, we show that Ca(2+)-regulated exocytosis of lysosomes is required for the repair of plasma membrane disruptions. Lysosomal exocytosis and membrane resealing are inhibited by the recombinant Syt VII C(2)A domain or anti-Syt VII C(2)A antibodies, or by antibodies against the cytosolic domain of Lamp-1, which specifically aggregate lysosomes. We further demonstrate that lysosomal exocytosis mediates the resealing of primary skin fibroblasts wounded during the contraction of collagen matrices. These findings reveal a fundamental, novel role for lysosomes: as Ca(2+)-regulated exocytic compartments responsible for plasma membrane repair.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0092-8674
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
27
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pubmed:volume |
106
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
157-69
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:11511344-Animals,
pubmed-meshheading:11511344-Antigens, CD,
pubmed-meshheading:11511344-Calcium,
pubmed-meshheading:11511344-Calcium-Binding Proteins,
pubmed-meshheading:11511344-Cell Division,
pubmed-meshheading:11511344-Cell Line,
pubmed-meshheading:11511344-Cell Membrane,
pubmed-meshheading:11511344-Cells, Cultured,
pubmed-meshheading:11511344-Collagen,
pubmed-meshheading:11511344-Cricetinae,
pubmed-meshheading:11511344-Exocytosis,
pubmed-meshheading:11511344-Fibroblasts,
pubmed-meshheading:11511344-Flow Cytometry,
pubmed-meshheading:11511344-Humans,
pubmed-meshheading:11511344-Lysosome-Associated Membrane Glycoproteins,
pubmed-meshheading:11511344-Lysosomes,
pubmed-meshheading:11511344-Membrane Glycoproteins,
pubmed-meshheading:11511344-Mice,
pubmed-meshheading:11511344-Microinjections,
pubmed-meshheading:11511344-Nerve Tissue Proteins,
pubmed-meshheading:11511344-Protein Structure, Tertiary,
pubmed-meshheading:11511344-Rats,
pubmed-meshheading:11511344-Regeneration,
pubmed-meshheading:11511344-Skin,
pubmed-meshheading:11511344-Synaptotagmins,
pubmed-meshheading:11511344-Wounds and Injuries
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pubmed:year |
2001
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pubmed:articleTitle |
Plasma membrane repair is mediated by Ca(2+)-regulated exocytosis of lysosomes.
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pubmed:affiliation |
Section of Microbial Pathogenesis, Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, CT 06520, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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