Source:http://linkedlifedata.com/resource/pubmed/id/11509837
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2001-8-17
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| pubmed:abstractText |
We have previously shown that pigment epithelium-derived factor (PEDF) acts as a survival factor for cerebellar granule cells (CGCs), by blocking apoptotic death, and can also protect these cells against glutamate-induced neurotoxicity. In preparation for gene therapy studies, pseudotyped HIV-1-based lentiviral vectors containing the PEDF gene, as well as either green fluorescent protein or beta-galactosidase, were prepared. These bicistronic vectors are unique in that they express two genes efficiently under one promoter. Primary cell cultures of CGCs from postnatal day 8 rats were infected with the vectors encoding PEDF. RT-PCR demonstrated expression of mRNA and Western blot analysis confirmed that infected CGCs secrete PEDF protein to the medium. Assays for cell survival demonstrated that PEDF-infected cells were significantly more protected compared with mock-infected controls for 6-8 days in culture, as well as against induced apoptosis. The PEDF vectors expressing tat (trans-acting transcription factor) provided more protection than the tat(-) vectors. These results demonstrate that while the lentiviral vectors expressing PEDF are as neuroprotective as the protein itself for CGCs, the vectors have the advantage of providing long-lasting expression of PEDF protein, which will be more effective in in vivo studies. The present results suggest that this system may be useful for gene therapy for neurodegenerative disorders.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Eye Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, tat,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Serpins,
http://linkedlifedata.com/resource/pubmed/chemical/pigment epithelium-derived factor
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| pubmed:status |
MEDLINE
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| pubmed:issn |
0378-5866
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2001 S. Karger AG, Basel
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| pubmed:issnType |
Print
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| pubmed:volume |
23
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
145-52
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| pubmed:dateRevised |
2006-4-21
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| pubmed:meshHeading |
pubmed-meshheading:11509837-Animals,
pubmed-meshheading:11509837-Apoptosis,
pubmed-meshheading:11509837-Blotting, Western,
pubmed-meshheading:11509837-Cell Survival,
pubmed-meshheading:11509837-Cells, Cultured,
pubmed-meshheading:11509837-Cerebellum,
pubmed-meshheading:11509837-Eye Proteins,
pubmed-meshheading:11509837-Gene Expression Regulation, Developmental,
pubmed-meshheading:11509837-Gene Expression Regulation, Viral,
pubmed-meshheading:11509837-Gene Products, tat,
pubmed-meshheading:11509837-Genetic Vectors,
pubmed-meshheading:11509837-Immunohistochemistry,
pubmed-meshheading:11509837-Lac Operon,
pubmed-meshheading:11509837-Lentivirus,
pubmed-meshheading:11509837-Nerve Growth Factors,
pubmed-meshheading:11509837-Proteins,
pubmed-meshheading:11509837-RNA, Messenger,
pubmed-meshheading:11509837-Rats,
pubmed-meshheading:11509837-Rats, Sprague-Dawley,
pubmed-meshheading:11509837-Serpins
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| pubmed:year |
2001
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| pubmed:articleTitle |
Survival effects of pigment epithelium-derived factor expressed by a lentiviral vector in rat cerebellar granule cells.
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| pubmed:affiliation |
Neurotrophic Factors Section, National Institute of Neurological Disorders and Stroke, NIH, Bethesda, Md. 20892-4126, USA.
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| pubmed:publicationType |
Journal Article
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