Linked Life Data

11509386

Source:http://linkedlifedata.com/resource/pubmed/id/11509386

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2001-8-17
pubmed:abstractText
In biological macromolecules, fluorophores often exhibit multiple depolarizing motions that require multiple lifetimes and rotational relaxation times to define fluorescence intensity and anisotropy decays. The related analysis of time-correlated single-photon counting data becomes uncertain due to the multitude of decay parameters and numerical sensitivity to deconvolution of the instrument response function (IRF) via discretization of integrals. By using simulations we show that improved discretizations based on quadratic and cubic local approximations of the IRF yield more accurate estimation of short rotational relaxation times and lifetimes than the commonly used Grinvald-Steinberg discretization, which in turn appears more reliable than two discretizations based on linear local approximations of the IRF. In addition, our simulation suggests that cubic approximation is the most advantageous in discriminating complex heterogeneous and homogeneous anisotropy decay. We show that among three different information criteria, the Akaike information criterion is best suited for detection of heterogeneity in rotational relaxation times. It is capable of detecting heterogeneity even when anisotropy decay appears homogeneous within statistical errors of estimation.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11509386-10460346, http://linkedlifedata.com/resource/pubmed/commentcorrection/11509386-10704313, http://linkedlifedata.com/resource/pubmed/commentcorrection/11509386-1467441, http://linkedlifedata.com/resource/pubmed/commentcorrection/11509386-1584038, http://linkedlifedata.com/resource/pubmed/commentcorrection/11509386-16997211, http://linkedlifedata.com/resource/pubmed/commentcorrection/11509386-19431735, http://linkedlifedata.com/resource/pubmed/commentcorrection/11509386-3111558, http://linkedlifedata.com/resource/pubmed/commentcorrection/11509386-3689871, http://linkedlifedata.com/resource/pubmed/commentcorrection/11509386-4579675, http://linkedlifedata.com/resource/pubmed/commentcorrection/11509386-4838786, http://linkedlifedata.com/resource/pubmed/commentcorrection/11509386-7729365, http://linkedlifedata.com/resource/pubmed/commentcorrection/11509386-8519969, http://linkedlifedata.com/resource/pubmed/commentcorrection/11509386-8785272, http://linkedlifedata.com/resource/pubmed/commentcorrection/11509386-8994617, http://linkedlifedata.com/resource/pubmed/commentcorrection/11509386-914824, http://linkedlifedata.com/resource/pubmed/commentcorrection/11509386-9788952
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0006-3495
pubmed:author
pubmed:issnType
Print
pubmed:volume
81
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1765-75
pubmed:dateRevised
2010-9-14
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
Complex homogeneous and heterogeneous fluorescence anisotropy decays: enhancing analysis accuracy.
pubmed:affiliation
Department of Biochemistry and Molecular Biology, Mayo Foundation, Rochester, Minnesota 55905, USA. bajzer@mayo.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't