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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2001-8-16
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pubmed:abstractText |
MN/CA9 is a cancer-related gene, frequently activated in human renal cell carcinomas (RCCs). To reveal the activation mechanism, we investigated the relationship between methylation status of the MN/CA9 promoter region and gene expression using 13 human RCCs, and examined the effect of in vitro CpG methylation on the MN/CA9 promoter activity using a human RCC cell line (SK-RC-44), expressing MN/CA9. MN/CA9 expression was evaluated by RT-PCR and observed in 10 of 13 RCCs (77%). A total of 9 out of 10 MN/CA9 -positive RCCs (90%) contained clear cell components. Methylation status of 6 CpGs in the MN/CA9 promoter region was decided by using the bisulfite genomic sequencing protocol. Out of 13 RCCs 9 (69%) showed partial hypomethylation of the CpG at -74 bp, while the other 4 RCCs and 3 normal kidney tissue samples showed complete methylation. Hypomethylation of the CpG at -74 bp was strongly correlated with MN/CA9 expression. Luciferase assay revealed that the MN/CA9 promoter activity was strongly suppressed by methylation of the CpG at -74 bp. These findings suggest that hypomethylation of the CpG at -74 bp in the MN/CA9 promoter region might play an important role in this gene activation of human RCC.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11506497-10430258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11506497-10551317,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11506497-10551812,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11506497-10574265,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11506497-10708480,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11506497-10760566,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11506497-2428759,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11506497-3323819,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11506497-6883509,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11506497-7543680,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11506497-7585152,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11506497-7937876,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11506497-8084592,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11506497-8104532,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11506497-8661007,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11506497-8692960,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11506497-9114053,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11506497-9163670,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11506497-9191010,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11506497-9230182,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11506497-9628328,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11506497-9665489,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11506497-9770531,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11506497-9813077,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11506497-9834236
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0007-0920
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2001 Cancer Research Campaign.
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pubmed:issnType |
Print
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pubmed:day |
17
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pubmed:volume |
85
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
563-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:11506497-Carcinoma, Renal Cell,
pubmed-meshheading:11506497-DNA, Neoplasm,
pubmed-meshheading:11506497-DNA Methylation,
pubmed-meshheading:11506497-DNA Primers,
pubmed-meshheading:11506497-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:11506497-Humans,
pubmed-meshheading:11506497-Kidney Neoplasms,
pubmed-meshheading:11506497-Promoter Regions, Genetic,
pubmed-meshheading:11506497-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:11506497-Transcriptional Activation,
pubmed-meshheading:11506497-Tumor Cells, Cultured,
pubmed-meshheading:11506497-Up-Regulation
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pubmed:year |
2001
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pubmed:articleTitle |
Hypomethylation of the MN/CA9 promoter and upregulated MN/CA9 expression in human renal cell carcinoma.
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pubmed:affiliation |
Department of Urology, Nara Medical University, 840 Shijo-cho, Kashihara, Nara 634-8522, Japan.
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pubmed:publicationType |
Journal Article
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