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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
16
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pubmed:dateCreated |
2001-8-15
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pubmed:abstractText |
Conditional expression of suicide genes in vivo has a wide range of applications in biological research and requires a minimal basal promoter activity in the uninduced state. To reduce basal activity of tetracycline (tc)-inducible target promoters we combined synthetic tet operators in varying numbers with a core promoter derived from the plant viral 35S promoter. An optimized promoter, P(TF), was found to exert a stringent regulation of luciferase in combination with tTA and rtTA in different mammalian cell lines. We linked P(TF) to the barnase gene, coding for a highly active RNase from Bacillus amyloliquefaciens. Stable cell clones expressing barnase under control of tTA exerted cell death only after tc withdrawal, correlating with a 10-fold induction of barnase mRNA expression. Directing tTA expression through a neuron-specific enolase promoter (P(NSE)) leads to barnase expression and cell death in neuronal cells after tc withdrawal. Taken together, our data demonstrate that a stringent control of barnase expression in the uninduced state improves cell ablation studies, as high frequencies of transgene propagation in both cell lines and in transgenic mice are observed.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11504884-10353625,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11504884-10809144,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11504884-10859354,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11504884-11254134,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11504884-1319065,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11504884-1467557,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11504884-2116814,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11504884-2696173,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11504884-7737125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11504884-7792603,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11504884-7937760,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11504884-8390389,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11504884-8824329,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11504884-8855286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11504884-9023121,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11504884-9338959,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11504884-9568712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11504884-9736743,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11504884-9863002
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1362-4962
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
15
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pubmed:volume |
29
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
E76
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pubmed:dateRevised |
2010-9-14
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pubmed:meshHeading |
pubmed-meshheading:11504884-Animals,
pubmed-meshheading:11504884-Bacillus,
pubmed-meshheading:11504884-Caulimovirus,
pubmed-meshheading:11504884-Cell Death,
pubmed-meshheading:11504884-Cell Line,
pubmed-meshheading:11504884-Enzyme Induction,
pubmed-meshheading:11504884-Genes, Reporter,
pubmed-meshheading:11504884-Genetic Vectors,
pubmed-meshheading:11504884-Humans,
pubmed-meshheading:11504884-Kinetics,
pubmed-meshheading:11504884-Mice,
pubmed-meshheading:11504884-Mice, Transgenic,
pubmed-meshheading:11504884-Nervous System,
pubmed-meshheading:11504884-Neurons,
pubmed-meshheading:11504884-Promoter Regions, Genetic,
pubmed-meshheading:11504884-RNA, Messenger,
pubmed-meshheading:11504884-Ribonucleases,
pubmed-meshheading:11504884-Tetracycline
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pubmed:year |
2001
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pubmed:articleTitle |
Conditional cell ablation by stringent tetracycline-dependent regulation of barnase in mammalian cells.
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pubmed:affiliation |
Developmental Biology and Molecular Pathology, W7, University of Bielefeld, 33615 Bielefeld, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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