Source:http://linkedlifedata.com/resource/pubmed/id/11504713
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
42
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| pubmed:dateCreated |
2001-10-15
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| pubmed:abstractText |
Pulmonary surfactant isolated from gene-targeted surfactant protein A null mice (SP-A(-/-)) is deficient in the surfactant aggregate tubular myelin and has surface tension-lowering activity that is easily inhibited by serum proteins in vitro. To further elucidate the role of SP-A and its collagen-like region in surfactant function, we used the human SP-C promoter to drive expression of rat SP-A (rSPA) or SP-A containing a deletion of the collagen-like domain (DeltaG8-P80) in the Clara cells and alveolar type II cells of SP-A(-/-) mice. The level of the SP-A in the alveolar wash of the SP-A(-/-,rSP-A) and SP-A(-/-,DeltaG8-P80) mice was 6.1-and 1.3-fold higher, respectively, than in the wild type controls. Tissue levels of saturated phosphatidylcholine were slightly reduced in the SP-A(-/-,rSP-A) mice compared with SP-A(-/-) littermates. Tubular myelin was present in the large surfactant aggregates isolated from the SP-A(-/-,rSP-A) lines but not in the SP-A(-/-,DeltaG8-P80) mice or SP-A(-/-) controls. The equilibrium and minimum surface tensions of surfactant from the SP-A(-/-,rSP-A) mice were similar to SP-A(-/-) controls, but both were markedly elevated in the SP-A(-/-,DeltaG8-P80) mice. There was no defect in the surface tension-lowering activity of surfactant from SP-A(+/+,DeltaG8-P80) mice, indicating that the inhibitory effect of DeltaG8-P80 on surface activity can be overcome by wild type levels of mouse SP-A. The surface activity of surfactant isolated from the SP-A(-/-,rSP-A) but not the SP-A(-/-,DeltaG8-P80) mice was more resistant than SP-A(-/-) littermate control animals to inhibition by serum proteins in vitro. Pressure volume relationships of lungs from the SP-A(-/-), SP-A(-/-,rSP-A), and SP-A(-/-,DeltaG8-P80) lines were very similar. These data indicate that expression of SP-A in the pulmonary epithelium of SP-A(-/-) animals restores tubular myelin formation and resistance of isolated surfactant to protein inhibition by a mechanism that is dependent on the collagen-like region.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Collagen,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylcholines,
http://linkedlifedata.com/resource/pubmed/chemical/Proteolipids,
http://linkedlifedata.com/resource/pubmed/chemical/Pulmonary Surfactant-Associated...,
http://linkedlifedata.com/resource/pubmed/chemical/Pulmonary Surfactant-Associated...,
http://linkedlifedata.com/resource/pubmed/chemical/Pulmonary Surfactants,
http://linkedlifedata.com/resource/pubmed/chemical/Surface-Active Agents
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| pubmed:status |
MEDLINE
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| pubmed:month |
Oct
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| pubmed:issn |
0021-9258
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
19
|
| pubmed:volume |
276
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
38542-8
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:11504713-Animals,
pubmed-meshheading:11504713-Blotting, Southern,
pubmed-meshheading:11504713-Collagen,
pubmed-meshheading:11504713-Gene Deletion,
pubmed-meshheading:11504713-Humans,
pubmed-meshheading:11504713-Immunoblotting,
pubmed-meshheading:11504713-Lung,
pubmed-meshheading:11504713-Mice,
pubmed-meshheading:11504713-Mice, Knockout,
pubmed-meshheading:11504713-Mice, Transgenic,
pubmed-meshheading:11504713-Microscopy, Electron,
pubmed-meshheading:11504713-Myelin Sheath,
pubmed-meshheading:11504713-Phosphatidylcholines,
pubmed-meshheading:11504713-Promoter Regions, Genetic,
pubmed-meshheading:11504713-Protein Structure, Tertiary,
pubmed-meshheading:11504713-Proteolipids,
pubmed-meshheading:11504713-Pulmonary Alveoli,
pubmed-meshheading:11504713-Pulmonary Surfactant-Associated Protein A,
pubmed-meshheading:11504713-Pulmonary Surfactant-Associated Proteins,
pubmed-meshheading:11504713-Pulmonary Surfactants,
pubmed-meshheading:11504713-Rats,
pubmed-meshheading:11504713-Surface Tension,
pubmed-meshheading:11504713-Surface-Active Agents,
pubmed-meshheading:11504713-Time Factors,
pubmed-meshheading:11504713-Transgenes
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| pubmed:year |
2001
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| pubmed:articleTitle |
The collagen-like region of surfactant protein A (SP-A) is required for correction of surfactant structural and functional defects in the SP-A null mouse.
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| pubmed:affiliation |
Division of Pulmonary and Critical Care Medicine, Department of Medicine, University of Cincinnati School of Medicine, Cincinnati, Ohio 45229-3039, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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