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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2001-8-13
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pubmed:abstractText |
Although it is known that transforming growth factor (TGF)-beta induces vascular endothelial growth factor (VEGF) synthesis in vascular smooth muscle cells, the underlying mechanisms are still poorly understood. In the present study, we examined whether the mitogen-activated protein (MAP) kinase superfamily is involved in TGF-beta-stimulated VEGF synthesis in aortic smooth muscle A10 cells. TGF-beta stimulated the phosphorylation of p42/p44 MAP kinase and p38 MAP kinase, but not that of SAPK (stress-activated protein kinase)/JNK (c-Jun N-terminal kinase). The VEGF synthesis induced by TGF-beta was not affected by PD98059 or U0126, specific inhibitors of the upstream kinase that activates p42/p44 MAP kinase. We confirmed that PD98059 or U0126 did actually suppress the phosphorylation of p42/p44 MAP kinase by TGF-beta in our preparations. PD169316 and SB203580, specific inhibitors of p38 MAP kinase, significantly reduced the TGF-beta-stimulated synthesis of VEGF (each in a dose-dependent manner). PD169316 or SB203580 attenuated the TGF-beta-induced phosphorylation of p38 MAP kinase. These results strongly suggest that p38 MAP kinase plays a part in the pathway by which TGF-beta stimulates the synthesis of VEGF in aortic smooth muscle cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Butadienes,
http://linkedlifedata.com/resource/pubmed/chemical/Endothelial Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Flavonoids,
http://linkedlifedata.com/resource/pubmed/chemical/Imidazoles,
http://linkedlifedata.com/resource/pubmed/chemical/JNK Mitogen-Activated Protein...,
http://linkedlifedata.com/resource/pubmed/chemical/Lymphokines,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase 1,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Nitriles,
http://linkedlifedata.com/resource/pubmed/chemical/PD 98059,
http://linkedlifedata.com/resource/pubmed/chemical/Pyridines,
http://linkedlifedata.com/resource/pubmed/chemical/SB 203580,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta,
http://linkedlifedata.com/resource/pubmed/chemical/U 0126,
http://linkedlifedata.com/resource/pubmed/chemical/Vascular Endothelial Growth Factor A,
http://linkedlifedata.com/resource/pubmed/chemical/Vascular Endothelial Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/p38 Mitogen-Activated Protein...
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pubmed:status |
MEDLINE
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pubmed:issn |
0730-2312
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2001 Wiley-Liss, Inc.
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pubmed:issnType |
Print
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pubmed:volume |
82
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
591-8
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:11500937-Animals,
pubmed-meshheading:11500937-Aorta,
pubmed-meshheading:11500937-Butadienes,
pubmed-meshheading:11500937-Cell Line,
pubmed-meshheading:11500937-Dose-Response Relationship, Drug,
pubmed-meshheading:11500937-Endothelial Growth Factors,
pubmed-meshheading:11500937-Flavonoids,
pubmed-meshheading:11500937-Imidazoles,
pubmed-meshheading:11500937-JNK Mitogen-Activated Protein Kinases,
pubmed-meshheading:11500937-Lymphokines,
pubmed-meshheading:11500937-Mitogen-Activated Protein Kinase 1,
pubmed-meshheading:11500937-Mitogen-Activated Protein Kinase 3,
pubmed-meshheading:11500937-Mitogen-Activated Protein Kinases,
pubmed-meshheading:11500937-Muscle, Smooth, Vascular,
pubmed-meshheading:11500937-Nitriles,
pubmed-meshheading:11500937-Phosphorylation,
pubmed-meshheading:11500937-Pyridines,
pubmed-meshheading:11500937-Rats,
pubmed-meshheading:11500937-Transforming Growth Factor beta,
pubmed-meshheading:11500937-Vascular Endothelial Growth Factor A,
pubmed-meshheading:11500937-Vascular Endothelial Growth Factors,
pubmed-meshheading:11500937-p38 Mitogen-Activated Protein Kinases
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pubmed:year |
2001
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pubmed:articleTitle |
Involvement of p38 MAP kinase in TGF-beta-stimulated VEGF synthesis in aortic smooth muscle cells.
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pubmed:affiliation |
Department of Pharmacology, Gifu University School of Medicine, Gifu, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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