Linked Life Data

11500825

Source:http://linkedlifedata.com/resource/pubmed/id/11500825

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2001-8-13
pubmed:abstractText
Despite increasing evidence for the role of the chemokine system in leukocyte trafficking, the mechanism underlying the induction of chemokine receptors is poorly understood. Here, we investigated how CCR5, a chemokine receptor implicated in T cell migration to inflammatory sites, is induced in the T cell. CCR5 mRNA was hardly detected in resting T cells and marginally induced following T cell receptor (TCR) stimulation. However, TCR-triggered T cells expressed IL-12 receptor, and stimulation with recombinant IL-12 resulted in high levels of CCR5 expression on both CD4(+) and CD8(+) T cells. In contrast, IL-2 failed to up-regulate CCR5 expression. The effect of IL-12 was selective to CCR5 because IL-12 did not up-regulate CXCR3 expression. Surface expression of CCR5 was shown by staining with anti-CCR5 monoclonal antibody. Stimulation of these CCR5-positive T cells with the relevant chemokine MIP-1 alpha elicited Ca(2+) influx, showing that IL-12-induced CCR5 is functional. These results indicate a critical role for IL-12 in the induction of CCR5 on TCR-triggered T cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0014-2980
pubmed:author
pubmed:issnType
Print
pubmed:volume
31
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2411-20
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:11500825-Animals, pubmed-meshheading:11500825-CD4-Positive T-Lymphocytes, pubmed-meshheading:11500825-CD8-Positive T-Lymphocytes, pubmed-meshheading:11500825-Calcium, pubmed-meshheading:11500825-Calcium Signaling, pubmed-meshheading:11500825-Cells, Cultured, pubmed-meshheading:11500825-Chemokine CCL4, pubmed-meshheading:11500825-Fluorescent Antibody Technique, pubmed-meshheading:11500825-Interferon-gamma, pubmed-meshheading:11500825-Interleukin-12, pubmed-meshheading:11500825-Macrophage Inflammatory Proteins, pubmed-meshheading:11500825-Mice, pubmed-meshheading:11500825-Mice, Inbred BALB C, pubmed-meshheading:11500825-RNA, Messenger, pubmed-meshheading:11500825-Receptors, Antigen, T-Cell, pubmed-meshheading:11500825-Receptors, CCR5, pubmed-meshheading:11500825-Up-Regulation
pubmed:year
2001
pubmed:articleTitle
A critical role for IL-12 in CCR5 induction on T cell receptor-triggered mouse CD4(+) and CD8(+) T cells.
pubmed:affiliation
Department of Oncology, Biomedical Research Center, Osaka University Graduate School of Medicine, Osaka, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't