Source:http://linkedlifedata.com/resource/pubmed/id/11499808
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
2001-8-13
|
| pubmed:abstractText |
We have isolated a 55 kDa protein from the seed extract of Aeginetia indica L. (AIL), a parasitic plant, by affinity chromatography on an N-hydroxysuccinimide-activated Sepharose High Performance column bound with F3, a monoclonal antibody that neutralizes the cytokine-inducing and anti-tumor effect of AIL. In the present study, we examined this protein (AILb-A) for cytokine induction and anti-tumor effects by animal study, using syngeneic Meth-A tumor-bearing BALB/c mice, in which the Th2 response is genetically dominant. AILb-A administration resulted in markedly increased levels of Th1 cytokines [interferon (IFN)-gamma, tumor necrosis factor (TNF)-alpha, interleukin (IL)-2, IL-12 and IL-18] in the sera derived from Meth-A-bearing mice. The in vitro re-stimulation with AILb-A of splenocytes derived from AILb-A-primed mice also selectively induced Th1-type cytokines and antigen-specific killer cell activity. The neutralizing test using cytokine-specific antibodies revealed that AILb-A-induced IL-18 plays a most significant role for and killer cell-inducing activities. Furthermore, IL-12 and IL-18 induced by AILb-A inhibited specifically IL-10 and IL-4 production, respectively. Finally, we examined the anti-tumor effect of AILb-A in both Meth-A-bearing BALB/c mice and Meth-A-bearing nude mice with BALB/c background. AILb-A exhibited a striking anti-tumor effect in normal BALB/c mice inoculated with Meth-A cells. In athymic nude mice, the anti-tumor effect of AILb-A was relatively weak. These findings strongly suggested that AILb-A is a potent Th1 inducer and may be a useful immunotherapeutic agent for patients with malignant diseases.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0340-7004
|
| pubmed:author |
pubmed-author:BandoTT,
pubmed-author:FuruichiSS,
pubmed-author:HimenoKK,
pubmed-author:NishikawaHH,
pubmed-author:OhkuboSS,
pubmed-author:OhrRR,
pubmed-author:OkamotoMM,
pubmed-author:OshikawaTT,
pubmed-author:SakaiTT,
pubmed-author:SatoMM,
pubmed-author:TanoTT,
pubmed-author:UyamaKK,
pubmed-author:YoshidaHH
|
| pubmed:issnType |
Print
|
| pubmed:volume |
50
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
251-9
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:11499808-Animals,
pubmed-meshheading:11499808-Antibodies,
pubmed-meshheading:11499808-Antineoplastic Agents, Phytogenic,
pubmed-meshheading:11499808-Cells, Cultured,
pubmed-meshheading:11499808-Cytokines,
pubmed-meshheading:11499808-Cytotoxicity Tests, Immunologic,
pubmed-meshheading:11499808-Female,
pubmed-meshheading:11499808-Fibrosarcoma,
pubmed-meshheading:11499808-Humans,
pubmed-meshheading:11499808-Mice,
pubmed-meshheading:11499808-Mice, Inbred BALB C,
pubmed-meshheading:11499808-Mice, Nude,
pubmed-meshheading:11499808-Neoplasms, Experimental,
pubmed-meshheading:11499808-Plant Proteins,
pubmed-meshheading:11499808-Spleen,
pubmed-meshheading:11499808-Survival Rate,
pubmed-meshheading:11499808-Th1 Cells,
pubmed-meshheading:11499808-Th2 Cells,
pubmed-meshheading:11499808-Tumor Cells, Cultured
|
| pubmed:year |
2001
|
| pubmed:articleTitle |
Th1-cytokine induction and anti-tumor effect of 55 kDa protein isolated from Aeginetia indica L., a parasitic plant.
|
| pubmed:affiliation |
Second Department of Oral and Maxillofacial Surgery, Tokushima University School of Dentistry, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|