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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
42
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pubmed:dateCreated |
2001-10-15
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pubmed:abstractText |
Rab11a is a small GTP-binding protein enriched in the pericentriolar plasma membrane recycling systems. We hypothesized that Rab11a-binding proteins exist as downstream effectors of its action. Here we define a family of four Rab11-interacting proteins: Rab11-Family Interacting Protein 1 (Rab11-FIP1), Rab11-Family Interacting Protein 2 (Rab11-FIP2), Rab11-Family Interacting Protein 3 (Rab11-FIP3), and pp75/Rip11. All four interacting proteins associated with wild type Rab11a and dominant active Rab11a (Rab11aS20V) as well as Rab11b and Rab25. Rab11-FIP2 also interacted with dominant negative Rab11a (Rab11aS25N) and the tail of myosin Vb. The binding of Rab11-FIP1, Rab11-FIP2, and Rab11-FIP3 to Rab11a was dependent upon a conserved carboxyl-terminal amphipathic alpha-helix. Rab11-FIP1, Rab11-FIP2, and pp75/Rip11 colocalized with Rab11a in plasma membrane recycling systems in both non-polarized HeLa cells and polarized Madin-Darby canine kidney cells. GFP-Rab11-FIP3 also colocalized with Rab11a in HeLa cells. Rab11-FIP1, Rab11-FIP2, and pp75/Rip11 also coenriched with Rab11a and H(+)K(+)-ATPase on parietal cell tubulovesicles, and Rab11-FIP1 and Rab11-FIP2 translocated with Rab11a and the H(+)K(+)-ATPase upon stimulating parietal cells with histamine. The results suggest that the function of Rab11a in plasma membrane recycling systems is dependent upon a compendium of protein effectors.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antineoplastic Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Antineoplastic Agents, Phytogenic,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Histamine,
http://linkedlifedata.com/resource/pubmed/chemical/Nocodazole,
http://linkedlifedata.com/resource/pubmed/chemical/Paclitaxel,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/rab GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/rab11 protein
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
19
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pubmed:volume |
276
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
39067-75
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:11495908-Amino Acid Sequence,
pubmed-meshheading:11495908-Animals,
pubmed-meshheading:11495908-Antineoplastic Agents,
pubmed-meshheading:11495908-Antineoplastic Agents, Phytogenic,
pubmed-meshheading:11495908-Base Sequence,
pubmed-meshheading:11495908-Blotting, Western,
pubmed-meshheading:11495908-Cell Line,
pubmed-meshheading:11495908-Cell Membrane,
pubmed-meshheading:11495908-Cloning, Molecular,
pubmed-meshheading:11495908-Conserved Sequence,
pubmed-meshheading:11495908-DNA, Complementary,
pubmed-meshheading:11495908-Dogs,
pubmed-meshheading:11495908-Expressed Sequence Tags,
pubmed-meshheading:11495908-Gastric Mucosa,
pubmed-meshheading:11495908-Gene Deletion,
pubmed-meshheading:11495908-Gene Library,
pubmed-meshheading:11495908-Genes, Dominant,
pubmed-meshheading:11495908-HeLa Cells,
pubmed-meshheading:11495908-Histamine,
pubmed-meshheading:11495908-Humans,
pubmed-meshheading:11495908-Microscopy, Fluorescence,
pubmed-meshheading:11495908-Molecular Sequence Data,
pubmed-meshheading:11495908-Nocodazole,
pubmed-meshheading:11495908-Paclitaxel,
pubmed-meshheading:11495908-Protein Binding,
pubmed-meshheading:11495908-Rabbits,
pubmed-meshheading:11495908-Recombinant Proteins,
pubmed-meshheading:11495908-Sequence Homology, Amino Acid,
pubmed-meshheading:11495908-Two-Hybrid System Techniques,
pubmed-meshheading:11495908-rab GTP-Binding Proteins
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pubmed:year |
2001
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pubmed:articleTitle |
Identification and characterization of a family of Rab11-interacting proteins.
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pubmed:affiliation |
Department of Medicine, Institute of Molecular Medicine and Genetics, Medical College of Georgia and the Augusta Veterans Affairs Medical Center, Augusta, Georgia 30912, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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