Linked Life Data

1149391

Source:http://linkedlifedata.com/resource/pubmed/id/1149391

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1975-10-30
pubmed:abstractText
1. A technique is described for the measurement of potassium and water spaces in less than 1 mul of packed cells. 2. The total potassium content of cell pellets is measured in a perchloric and nitric acid extract by flame spectrophotometry. The potassium in trapped medium is estimated from the distribution space of hydroxy[-14C]methylinulin and subtracted from the total potassium content to give intracellular potassium content. Corticosterone output was measured by radioimmunoassay. 3. The simultaneous measurement of total water from the [-3H]water space allows calculation of the intracellular water space. 4. Values obtained for intracellular potassium content, intracellular water space and calculated intracellular potassium concentration are presented for different preparations of isolated adrenal cortical cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0301-0538
pubmed:author
pubmed:issnType
Print
pubmed:volume
49
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1-12
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1975
pubmed:articleTitle
Micromethods for the simultaneous measurement of intracellular potassium and corticosterone output of isolated adrenal cortical cells.
pubmed:publicationType
Journal Article, In Vitro