Linked Life Data

11491349

Source:http://linkedlifedata.com/resource/pubmed/id/11491349

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 4
pubmed:dateCreated
2001-8-8
pubmed:databankReference
pubmed:abstractText
A polyphasic taxonomic study, including amplified fragment length polymorphism (AFLP) fingerprinting, DNA-DNA hybridizations, DNA base-ratio determinations, phylogenetic analysis, whole-cell fatty acid analyses and an extensive biochemical characterization, was performed on 19 Burkholderia cepacia-like isolates from the environment and cystic fibrosis (CF) patients. Several of the environmental isolates have attracted considerable interest due to their biocontrol properties. The polyphasic taxonomic data showed that the strains represent a new member of the B. cepacia complex, for which the name Burkholderia ambifaria sp. nov. is proposed. The type strain is strain LMG 19182T. B. ambifaria can be differentiated from the other members of the B. cepacia complex by means of AFLP fingerprinting, whole-cell fatty acid analysis, biochemical tests (including ornithine and lysine decarboxylase activity, acidification of sucrose and beta-haemolysis) and a newly developed recA gene-based PCR assay. 16S rDNA-based RFLP analysis and PCR tests allowed differentiation of B. ambifaria from Burkholderia multivorans, Burkholderia vietnamiensis and B. cepacia genomovar VI, but not from B. cepacia genomovars I and III and Burkholderia stabilis. The finding that this new taxon includes both strains isolated from CF patients and potentially useful biocontrol strains supports the general consensus that the large-scale use of biocontrol strains belonging to the B. cepacia complex would be ill-advised until more is known about their potential pathogenic mechanisms.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1466-5026
pubmed:author
pubmed:issnType
Print
pubmed:volume
51
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1481-90
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11491349-Base Composition, pubmed-meshheading:11491349-Burkholderia, pubmed-meshheading:11491349-Burkholderia cepacia, pubmed-meshheading:11491349-Cystic Fibrosis, pubmed-meshheading:11491349-DNA, Bacterial, pubmed-meshheading:11491349-DNA, Ribosomal, pubmed-meshheading:11491349-DNA Fingerprinting, pubmed-meshheading:11491349-Environmental Microbiology, pubmed-meshheading:11491349-Fatty Acids, pubmed-meshheading:11491349-Humans, pubmed-meshheading:11491349-Molecular Sequence Data, pubmed-meshheading:11491349-Nucleic Acid Hybridization, pubmed-meshheading:11491349-Phenotype, pubmed-meshheading:11491349-Phylogeny, pubmed-meshheading:11491349-Polymorphism, Genetic, pubmed-meshheading:11491349-Polymorphism, Restriction Fragment Length, pubmed-meshheading:11491349-Species Specificity
pubmed:year
2001
pubmed:articleTitle
Burkholderia ambifaria sp. nov., a novel member of the Burkholderia cepacia complex including biocontrol and cystic fibrosis-related isolates.
pubmed:affiliation
Laboratorium voor Microbiologie, Universiteit Gent, Ghent, Belgium. Tom.Coenye@rug.ac.be
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't