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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2001-8-7
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pubmed:abstractText |
Long-term cultured pre-B cells are able to differentiate into immunoglobulin (Ig)M-positive B cells (IgM(+) cells) when transplanted into severe combined immunodeficient (SCID) mice. Based on previous studies, here we report the development of a reconstitution assay in nonobese diabetic/SCID (NOD/SCID) mice using pre-B cells, which allows us to study the role of calpains (calcium-activated endopeptidases) during B cell development as well as in B cell clonal deletion. Using this model, we show that calpastatin (the natural inhibitor of calpains) inhibits B cell receptor-induced apoptosis in IgM(+) cells derived from transplanted mice. We thus hypothesize an important function for calpain in sculpting the B cell repertoire.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11489944-10022537,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11489944-10029585,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11489944-10069958,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11489944-10224236,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11489944-10403772,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11489944-10438500,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11489944-10487751,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11489944-10515867,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11489944-10527459,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11489944-10601010,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11489944-10620600,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11489944-10631785,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11489944-10830966,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11489944-11043765,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11489944-11193029,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11489944-1402680,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11489944-1847517,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11489944-1909664,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11489944-2050681,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11489944-2057527,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11489944-2989695,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11489944-7699333,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11489944-7821418,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11489944-7989752,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11489944-7995938,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11489944-8228816,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11489944-8250833,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11489944-8293461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11489944-8442861,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11489944-8764092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11489944-9233618,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11489944-9244303,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11489944-9558076,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11489944-9642102,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11489944-9795130,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11489944-9823889
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0022-1007
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
6
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pubmed:volume |
194
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
247-54
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:11489944-Animals,
pubmed-meshheading:11489944-Apoptosis,
pubmed-meshheading:11489944-B-Lymphocytes,
pubmed-meshheading:11489944-Calcium-Binding Proteins,
pubmed-meshheading:11489944-Calpain,
pubmed-meshheading:11489944-Cell Differentiation,
pubmed-meshheading:11489944-Cell Line,
pubmed-meshheading:11489944-Clonal Deletion,
pubmed-meshheading:11489944-Gene Expression,
pubmed-meshheading:11489944-Hematopoiesis,
pubmed-meshheading:11489944-Hematopoietic Stem Cell Transplantation,
pubmed-meshheading:11489944-Hematopoietic Stem Cells,
pubmed-meshheading:11489944-Immunoglobulin M,
pubmed-meshheading:11489944-Interleukin-7,
pubmed-meshheading:11489944-Mice,
pubmed-meshheading:11489944-Mice, Inbred NOD,
pubmed-meshheading:11489944-Mice, SCID,
pubmed-meshheading:11489944-Models, Biological,
pubmed-meshheading:11489944-Receptors, Antigen, B-Cell
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pubmed:year |
2001
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pubmed:articleTitle |
Transplanted long-term cultured pre-BI cells expressing calpastatin are resistant to B cell receptor-induced apoptosis.
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pubmed:affiliation |
Department of Immunology and Oncology, Centro Nacional de Biotecnología/CSIC, Universidad Autónoma de Madrid, Campus de Cantoblanco UAM, E-28049 Madrid, Spain. aruiz@cnb.uam.es
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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