Linked Life Data

11489121

Source:http://linkedlifedata.com/resource/pubmed/id/11489121

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2001-8-7
pubmed:databankReference
pubmed:abstractText
Phage-host interactions remain poorly understood in lactic acid bacteria and essentially in all Gram-positive bacteria. The aim of this study was to identify the phage genetic determinant (anti-receptor) involved in the recognition of Streptococcus thermophilus hosts. The complete genomic sequence of the lytic S. thermophilus phage DT1 was determined previously, and bioinformatic analysis indicated that orf18 might be the anti-receptor gene. The orf18 of six additional S. thermophilus phages was determined (DT2, DT4, MD1, MD2, MD4 and Q5) and compared with the orf18 of DT1. The deduced ORF18 was divided into three domains. The first domain, which contains the N-terminal part of the protein, was conserved in all seven phages. The second domain was detected in only two phages and flanked by a motif called collagen-like repeats. The second domain also contained a variable region (VR1). All seven phages had a third domain that consisted of the C-terminal section of the protein as well as another variable region (VR2). Chimeric DT1 phages were constructed by recombination; a portion of its orf18 was replaced by the corresponding section in orf18 of the phage MD4. All DT1 chimeric phages acquired the host range of phage MD4. Analysis of the orf18 in the chimeric phages revealed that host specificity in phages DT1 and MD4 resulted from VR2. This is the first report on the identification and characterization of a phage gene involved in the host recognition process of Gram-positive bacteria.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0950-382X
pubmed:author
pubmed:issnType
Print
pubmed:volume
41
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
325-36
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:11489121-Adsorption, pubmed-meshheading:11489121-Amino Acid Sequence, pubmed-meshheading:11489121-Base Sequence, pubmed-meshheading:11489121-Computational Biology, pubmed-meshheading:11489121-DNA, Recombinant, pubmed-meshheading:11489121-Databases, Genetic, pubmed-meshheading:11489121-Genes, Viral, pubmed-meshheading:11489121-Host-Parasite Interactions, pubmed-meshheading:11489121-Molecular Sequence Data, pubmed-meshheading:11489121-Open Reading Frames, pubmed-meshheading:11489121-Protein Structure, Tertiary, pubmed-meshheading:11489121-Recombination, Genetic, pubmed-meshheading:11489121-Repetitive Sequences, Amino Acid, pubmed-meshheading:11489121-Sequence Alignment, pubmed-meshheading:11489121-Sequence Homology, Amino Acid, pubmed-meshheading:11489121-Streptococcus, pubmed-meshheading:11489121-Streptococcus Phages, pubmed-meshheading:11489121-Substrate Specificity
pubmed:year
2001
pubmed:articleTitle
Identification of a genetic determinant responsible for host specificity in Streptococcus thermophilus bacteriophages.
pubmed:affiliation
Département de Biochimie et de Microbiologie, Faculté des Sciences et de Génie, Université Laval, Québec, Canada, G1K 7P4.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't