Source:http://linkedlifedata.com/resource/pubmed/id/11488639
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2001-8-7
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| pubmed:abstractText |
We recently demonstrated that IgG from patients with systemic lupus erythematosus (SLE) in combination with U937 cells made apoptotic by UV-irradiation, can induce interferon-alpha (IFN-alpha) production in normal peripheral blood mononuclear cells (PBMC). In the present study we show by flow cytometry that the actual IFN-alpha producing cells (IPC) among PBMC had the same phenotype (HLA-DR+, CD4+, CD11b-, CD11c-, CD14-, CD19-, CD32-, CD36+, CD40+, CD45RA+, CD68+, CD83+, CD86-, IL-3R+ and IL-10R-) and low frequency (approximately 2/10(4)PBMC) as the IPC activated by Herpes simplex virus type I. Consequently, these cells correspond to the natural IPC, also described as type 2 precursor dendritic cells. We also demonstrated that cytokines of possible importance in the pathogenesis in SLE had effects on the IFN-alpha production. Specifically, the IFN-alpha production was strongly increased by the type I IFNs, IFN-alpha and -beta, but markedly inhibited by IL-10 and also to some extent by TFN-alpha. In contrast, the cytokines IFN-gamma, IL-6, TGF-beta and GM-CSF had no clear effects. No production of IL-10 was detected in PBMC stimulated by apoptotic U937 cells and SLE IgG. These results may explain the cause of the ongoing IFN-alpha production in SLE patients and its relation to the autoimmune process.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Autoantibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-10
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| pubmed:status |
MEDLINE
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| pubmed:month |
Aug
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| pubmed:issn |
0896-8411
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2001 Academic Press.
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| pubmed:issnType |
Print
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| pubmed:volume |
17
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
71-80
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| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:11488639-Adolescent,
pubmed-meshheading:11488639-Aged,
pubmed-meshheading:11488639-Apoptosis,
pubmed-meshheading:11488639-Autoantibodies,
pubmed-meshheading:11488639-Cells, Cultured,
pubmed-meshheading:11488639-Cytokines,
pubmed-meshheading:11488639-Female,
pubmed-meshheading:11488639-Flow Cytometry,
pubmed-meshheading:11488639-Humans,
pubmed-meshheading:11488639-Immunoglobulin G,
pubmed-meshheading:11488639-Immunophenotyping,
pubmed-meshheading:11488639-Interferon-alpha,
pubmed-meshheading:11488639-Interleukin-10,
pubmed-meshheading:11488639-Leukocytes, Mononuclear,
pubmed-meshheading:11488639-Lupus Vulgaris,
pubmed-meshheading:11488639-Male,
pubmed-meshheading:11488639-Scattering, Radiation,
pubmed-meshheading:11488639-U937 Cells,
pubmed-meshheading:11488639-Ultraviolet Rays
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| pubmed:year |
2001
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| pubmed:articleTitle |
Activation of natural interferon-alpha producing cells by apoptotic U937 cells combined with lupus IgG and its regulation by cytokines.
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| pubmed:affiliation |
Department of Medical Sciences, Section of Rheumatology, University Hospital, Uppsala, Sweden. ullvi.bave@medsci.uu.se
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|