Linked Life Data

11488301

Source:http://linkedlifedata.com/resource/pubmed/id/11488301

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2001-8-6
pubmed:abstractText
32P-Labeled fat bodies (32P-fat bodies) of Rhodnius prolixus females were incubated in the presence of non radioactive purified lipophorin and the release of radioactivity to the medium was analysed to answer the question of whether lipophorin is a reusable shuttle for phospholipids. The radioactivity found in the medium was associated with lipophorin phospholipids. When the 32P-fat bodies were incubated in the absence of lipophorin, only a small amount of radioactivity was released and it was not associated with lipophorin, indicating that there was no release of pre-labeled 32P-lipophorin by the tissue. Analysis of 32P-phospholipids transferred from fat bodies to the lipophorin particles by thin-layer chromatography revealed a predominance of phosphatidylethanolamine and phosphatidylcholine, with minor amounts of phosphatidylserine, phosphatidylinositol, and sphingomyelin. The transfer of phospholipids to lipophorin was linear with time up to 45 min and the process was inhibited at low temperature and by the metabolic inhibitors azide and fluoride. The transfer of phospholipids from the fat bodies to lipophorin was saturable with respect to the concentration of lipophorin, which was half-maximal at about 8 mg/ml. A directional movement of phospholipids from the fat body to lipophorin was observed. The net gain of phospholipids in 2 h of incubation with fat body was 8.54 nmol per insect, which corresponds to 6.69% of increase in the lipophorin phospholipid content. The rate of 32P-phospholipid transfer from fat body to lipophorin particles varied during the days after a blood meal increasing up to day 10 and then decreasing in parallel with the process of oogenesis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0739-4462
pubmed:author
pubmed:issnType
Print
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
133-44
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11488301-Animals, pubmed-meshheading:11488301-Autoradiography, pubmed-meshheading:11488301-Azides, pubmed-meshheading:11488301-Biological Transport, pubmed-meshheading:11488301-Carrier Proteins, pubmed-meshheading:11488301-Electrophoresis, Polyacrylamide Gel, pubmed-meshheading:11488301-Fat Body, pubmed-meshheading:11488301-Female, pubmed-meshheading:11488301-Fluorides, pubmed-meshheading:11488301-Hemolymph, pubmed-meshheading:11488301-Insect Proteins, pubmed-meshheading:11488301-Intestines, pubmed-meshheading:11488301-Lipoproteins, pubmed-meshheading:11488301-Oogenesis, pubmed-meshheading:11488301-Phosphatidylcholines, pubmed-meshheading:11488301-Phosphatidylethanolamines, pubmed-meshheading:11488301-Phospholipids, pubmed-meshheading:11488301-Phosphorus Radioisotopes, pubmed-meshheading:11488301-Rabbits, pubmed-meshheading:11488301-Rhodnius, pubmed-meshheading:11488301-Time Factors, pubmed-meshheading:11488301-Vitellogenins
pubmed:year
1992
pubmed:articleTitle
Transfer of phospholipids from fat body to lipophorin in Rhodnius prolixus.
pubmed:affiliation
Departamento de Bioquímica Médica, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, RJ, Brasil.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't