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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
16
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pubmed:dateCreated |
2001-8-6
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pubmed:abstractText |
Systemic administration of the glutamic acid analog kainic acid (KA) causes neuronal cell death in brain-vulnerable regions, such as the piriform cortex, hippocampus, and amygdala in rats. We investigated the relationship between the KA-induced neuronal apoptosis and expression of cyclin-dependent kinase 4 (CDK4) and cyclin D1, key regulators of cell cycle progression. Expression of CDK4 and cyclin D1 was upregulated in neurons of the rat piriform cortex and amygdala 1-3 d after KA administration in vivo. CDK4 and cyclin D1 proteins were induced in the cytoplasm and nuclei of neurons, with a concomitant increase of CDK4- and cyclin D1-positive microglia in the affected areas. Continuous infusion of 100 microm CDK4 or cyclin D1 antisense oligonucleotides into the lateral ventricle using mini-osmotic pumps suppressed the excitotoxin-induced neuronal cell death in the piriform cortex and basolateral amygdaloid nucleus, whereas sense oligonucleotides exhibited no such effect. Although KA administration causes prolonged c-Fos expression in the vulnerable regions that preceded the induction of neuronal apoptosis, the CDK4 or cyclin D1 antisense oligonucleotides exhibited no suppressive effect on c-Fos levels. Our results suggest that CDK4 and cyclin D1 are essential for KA-induced neuronal apoptosis in vivo.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation,
http://linkedlifedata.com/resource/pubmed/chemical/Cdk4 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin D1,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinase 4,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Kainic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides, Antisense,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-fos,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1529-2401
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
15
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pubmed:volume |
21
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6086-94
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:11487632-Amygdala,
pubmed-meshheading:11487632-Animals,
pubmed-meshheading:11487632-Antigens, Differentiation,
pubmed-meshheading:11487632-Apoptosis,
pubmed-meshheading:11487632-Cerebral Cortex,
pubmed-meshheading:11487632-Cyclin D1,
pubmed-meshheading:11487632-Cyclin-Dependent Kinase 4,
pubmed-meshheading:11487632-Cyclin-Dependent Kinases,
pubmed-meshheading:11487632-Hippocampus,
pubmed-meshheading:11487632-In Situ Hybridization,
pubmed-meshheading:11487632-In Situ Nick-End Labeling,
pubmed-meshheading:11487632-Injections, Intraventricular,
pubmed-meshheading:11487632-Kainic Acid,
pubmed-meshheading:11487632-Male,
pubmed-meshheading:11487632-Microglia,
pubmed-meshheading:11487632-Neurons,
pubmed-meshheading:11487632-Olfactory Pathways,
pubmed-meshheading:11487632-Oligonucleotides, Antisense,
pubmed-meshheading:11487632-Proto-Oncogene Proteins,
pubmed-meshheading:11487632-Proto-Oncogene Proteins c-fos,
pubmed-meshheading:11487632-RNA, Messenger,
pubmed-meshheading:11487632-Rats,
pubmed-meshheading:11487632-Rats, Sprague-Dawley,
pubmed-meshheading:11487632-Up-Regulation
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pubmed:year |
2001
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pubmed:articleTitle |
Cyclin-dependent kinase 4 and cyclin D1 are required for excitotoxin-induced neuronal cell death in vivo.
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pubmed:affiliation |
Department of Neurobiology (C1), Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan. ino@med.m.chiba-u.ac.jp
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pubmed:publicationType |
Journal Article
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