| pubmed-article:11485830 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:11485830 | lifeskim:mentions | umls-concept:C0007589 | lld:lifeskim |
| pubmed-article:11485830 | lifeskim:mentions | umls-concept:C0282549 | lld:lifeskim |
| pubmed-article:11485830 | lifeskim:mentions | umls-concept:C0033634 | lld:lifeskim |
| pubmed-article:11485830 | lifeskim:mentions | umls-concept:C1879547 | lld:lifeskim |
| pubmed-article:11485830 | lifeskim:mentions | umls-concept:C1511938 | lld:lifeskim |
| pubmed-article:11485830 | lifeskim:mentions | umls-concept:C1719914 | lld:lifeskim |
| pubmed-article:11485830 | lifeskim:mentions | umls-concept:C2828406 | lld:lifeskim |
| pubmed-article:11485830 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:11485830 | pubmed:dateCreated | 2001-8-3 | lld:pubmed |
| pubmed-article:11485830 | pubmed:abstractText | The human myelocytic leukemia cell line HL-60 is a useful model for the study of cellular differentiation. Phorbol 12-myristate 13-acetate (PMA) induces the monocyte/macrophage-like differentiation of HL-60 cells and results in growth arrest, increasing adherence. In PMA-induced differentiation of HL-60 cells, phosphoinositide 3-kinase (PI 3-K) activity was measured as phosphatidylinositol3P recovery from phosphatidylinositol by in vitro kinase assay. PI 3-K activity was increased in HL-60 cells that were stimulated by 20 nM PMA and the activity was inhibited by pretreatment with 20 microM LY294002, a specific inhibitor of PI 3-K. Members of the protein kinase C (PKC) family have been suggested to be one of the downstream targets of PI 3-K. PKC zeta is one of the atypical PKCs, non-diacylglycerol-responsive PKCs, and the activity was measured by the ability of phosphorylation onto myelin basic protein. PMA also induced the activation of PKC zeta during monocytic differentiation of HL-60 cells, and LY294002-pretreated cells failed to induce PKC zeta activation. The activity of PI 3-K is essential for PKC zeta activation, and LY294002 blocks both monocytic differentiation of HL-60 cells and activation of PKC zeta during PMA-induced cell differentiation. This implies that activated PI 3-K subsequently stimulates the PKC zeta in the process of PMA-induced monocytic differentiation. | lld:pubmed |
| pubmed-article:11485830 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11485830 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11485830 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:11485830 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11485830 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11485830 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11485830 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11485830 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11485830 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11485830 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11485830 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11485830 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11485830 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11485830 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:11485830 | pubmed:issn | 0304-3835 | lld:pubmed |
| pubmed-article:11485830 | pubmed:author | pubmed-author:LinW LWL | lld:pubmed |
| pubmed-article:11485830 | pubmed:author | pubmed-author:KimM SMS | lld:pubmed |
| pubmed-article:11485830 | pubmed:author | pubmed-author:ParkJ HJH | lld:pubmed |
| pubmed-article:11485830 | pubmed:author | pubmed-author:LeeY MYM | lld:pubmed |
| pubmed-article:11485830 | pubmed:author | pubmed-author:LalK BKB | lld:pubmed |
| pubmed-article:11485830 | pubmed:author | pubmed-author:LIMR KRK | lld:pubmed |
| pubmed-article:11485830 | pubmed:author | pubmed-author:YooS JSJ | lld:pubmed |
| pubmed-article:11485830 | pubmed:author | pubmed-author:AnN HNH | lld:pubmed |
| pubmed-article:11485830 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11485830 | pubmed:day | 28 | lld:pubmed |
| pubmed-article:11485830 | pubmed:volume | 171 | lld:pubmed |
| pubmed-article:11485830 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11485830 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11485830 | pubmed:pagination | 79-85 | lld:pubmed |
| pubmed-article:11485830 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
| pubmed-article:11485830 | pubmed:meshHeading | pubmed-meshheading:11485830... | lld:pubmed |
| pubmed-article:11485830 | pubmed:meshHeading | pubmed-meshheading:11485830... | lld:pubmed |
| pubmed-article:11485830 | pubmed:meshHeading | pubmed-meshheading:11485830... | lld:pubmed |
| pubmed-article:11485830 | pubmed:meshHeading | pubmed-meshheading:11485830... | lld:pubmed |
| pubmed-article:11485830 | pubmed:meshHeading | pubmed-meshheading:11485830... | lld:pubmed |
| pubmed-article:11485830 | pubmed:meshHeading | pubmed-meshheading:11485830... | lld:pubmed |
| pubmed-article:11485830 | pubmed:meshHeading | pubmed-meshheading:11485830... | lld:pubmed |
| pubmed-article:11485830 | pubmed:meshHeading | pubmed-meshheading:11485830... | lld:pubmed |
| pubmed-article:11485830 | pubmed:meshHeading | pubmed-meshheading:11485830... | lld:pubmed |
| pubmed-article:11485830 | pubmed:meshHeading | pubmed-meshheading:11485830... | lld:pubmed |
| pubmed-article:11485830 | pubmed:meshHeading | pubmed-meshheading:11485830... | lld:pubmed |
| pubmed-article:11485830 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11485830 | pubmed:articleTitle | The activation of PI 3-K and PKC zeta in PMA-induced differentiation of HL-60 cells. | lld:pubmed |
| pubmed-article:11485830 | pubmed:affiliation | College of Pharmacy and Center of Oriental Medicinal Science, Wonkwang University, Iksan, 570-749, Chonbuk, South Korea. | lld:pubmed |
| pubmed-article:11485830 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11485830 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11485830 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:11485830 | lld:pubmed |
