Source:http://linkedlifedata.com/resource/pubmed/id/11485830
Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
|
pubmed:dateCreated |
2001-8-3
|
pubmed:abstractText |
The human myelocytic leukemia cell line HL-60 is a useful model for the study of cellular differentiation. Phorbol 12-myristate 13-acetate (PMA) induces the monocyte/macrophage-like differentiation of HL-60 cells and results in growth arrest, increasing adherence. In PMA-induced differentiation of HL-60 cells, phosphoinositide 3-kinase (PI 3-K) activity was measured as phosphatidylinositol3P recovery from phosphatidylinositol by in vitro kinase assay. PI 3-K activity was increased in HL-60 cells that were stimulated by 20 nM PMA and the activity was inhibited by pretreatment with 20 microM LY294002, a specific inhibitor of PI 3-K. Members of the protein kinase C (PKC) family have been suggested to be one of the downstream targets of PI 3-K. PKC zeta is one of the atypical PKCs, non-diacylglycerol-responsive PKCs, and the activity was measured by the ability of phosphorylation onto myelin basic protein. PMA also induced the activation of PKC zeta during monocytic differentiation of HL-60 cells, and LY294002-pretreated cells failed to induce PKC zeta activation. The activity of PI 3-K is essential for PKC zeta activation, and LY294002 blocks both monocytic differentiation of HL-60 cells and activation of PKC zeta during PMA-induced cell differentiation. This implies that activated PI 3-K subsequently stimulates the PKC zeta in the process of PMA-induced monocytic differentiation.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2-(4-morpholinyl)-8-phenyl-4H-1-benz...,
http://linkedlifedata.com/resource/pubmed/chemical/Chromones,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Morpholines,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol 3-Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate,
http://linkedlifedata.com/resource/pubmed/chemical/protein kinase C zeta
|
pubmed:status |
MEDLINE
|
pubmed:month |
Sep
|
pubmed:issn |
0304-3835
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
28
|
pubmed:volume |
171
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
79-85
|
pubmed:dateRevised |
2010-11-18
|
pubmed:meshHeading |
pubmed-meshheading:11485830-Cell Differentiation,
pubmed-meshheading:11485830-Chromones,
pubmed-meshheading:11485830-Enzyme Activation,
pubmed-meshheading:11485830-Enzyme Inhibitors,
pubmed-meshheading:11485830-HL-60 Cells,
pubmed-meshheading:11485830-Humans,
pubmed-meshheading:11485830-Morpholines,
pubmed-meshheading:11485830-Neoplasm Proteins,
pubmed-meshheading:11485830-Phosphatidylinositol 3-Kinases,
pubmed-meshheading:11485830-Protein Kinase C,
pubmed-meshheading:11485830-Tetradecanoylphorbol Acetate
|
pubmed:year |
2001
|
pubmed:articleTitle |
The activation of PI 3-K and PKC zeta in PMA-induced differentiation of HL-60 cells.
|
pubmed:affiliation |
College of Pharmacy and Center of Oriental Medicinal Science, Wonkwang University, Iksan, 570-749, Chonbuk, South Korea.
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|