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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2001-8-3
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pubmed:abstractText |
SV40 large T-antigen-transfected bovine pulp-derived cells were grown three-dimensionally on polyamide meshes. For optimal cell growth, various cell numbers and mesh coatings were tested. Next the three-dimensional cultures were used in a dentin barrier test device, and the system was evaluated by testing a set of dental filling materials. After 24 hr exposure with or without perfusion of the pulpal part of the test device, cell survival was evaluated using an MTT assay. In all experiments pulp-derived cells transfected with SV40 large T-antigen grew three-dimensionally on polyamide meshes and showed growth kinetics similar to those on cell culture plates with lag, log, and plateau phases (reached after about 14 days of incubation). Cross-sections of the three-dimensional cell cultures revealed about 15 to 20 cell layers. In vitro cytotoxicity tests resulted in cell survival rates which are in good agreement with in vivo data and with results obtained from cytotoxicity tests with three-dimensional cultures of human foreskin fibroblasts.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
D
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Polyomavirus Transforming,
http://linkedlifedata.com/resource/pubmed/chemical/Coloring Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media,
http://linkedlifedata.com/resource/pubmed/chemical/Phenol,
http://linkedlifedata.com/resource/pubmed/chemical/Root Canal Filling Materials,
http://linkedlifedata.com/resource/pubmed/chemical/Tetrazolium Salts,
http://linkedlifedata.com/resource/pubmed/chemical/Thiazoles,
http://linkedlifedata.com/resource/pubmed/chemical/Tolonium Chloride,
http://linkedlifedata.com/resource/pubmed/chemical/Zinc Oxide-Eugenol Cement,
http://linkedlifedata.com/resource/pubmed/chemical/Zinc Phosphate Cement,
http://linkedlifedata.com/resource/pubmed/chemical/thiazolyl blue
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0099-2399
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
27
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
259-65
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pubmed:dateRevised |
2005-11-17
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pubmed:meshHeading |
pubmed-meshheading:11485263-Animals,
pubmed-meshheading:11485263-Antigens, Polyomavirus Transforming,
pubmed-meshheading:11485263-Cattle,
pubmed-meshheading:11485263-Cell Count,
pubmed-meshheading:11485263-Cell Division,
pubmed-meshheading:11485263-Cell Survival,
pubmed-meshheading:11485263-Cells, Cultured,
pubmed-meshheading:11485263-Coloring Agents,
pubmed-meshheading:11485263-Culture Media,
pubmed-meshheading:11485263-Dental Pulp,
pubmed-meshheading:11485263-Dentin,
pubmed-meshheading:11485263-Diffusion Chambers, Culture,
pubmed-meshheading:11485263-Fibroblasts,
pubmed-meshheading:11485263-Humans,
pubmed-meshheading:11485263-Perfusion,
pubmed-meshheading:11485263-Phenol,
pubmed-meshheading:11485263-Root Canal Filling Materials,
pubmed-meshheading:11485263-Sensitivity and Specificity,
pubmed-meshheading:11485263-Skin,
pubmed-meshheading:11485263-Statistics, Nonparametric,
pubmed-meshheading:11485263-Tetrazolium Salts,
pubmed-meshheading:11485263-Thiazoles,
pubmed-meshheading:11485263-Time Factors,
pubmed-meshheading:11485263-Tolonium Chloride,
pubmed-meshheading:11485263-Transfection,
pubmed-meshheading:11485263-Zinc Oxide-Eugenol Cement,
pubmed-meshheading:11485263-Zinc Phosphate Cement
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pubmed:year |
2001
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pubmed:articleTitle |
Cytotoxicity testing with three-dimensional cultures of transfected pulp-derived cells.
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pubmed:affiliation |
Department of Operative Dentistry and Periodontology, University of Regensburg, Germany.
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pubmed:publicationType |
Journal Article
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