Linked Life Data

11485197

Source:http://linkedlifedata.com/resource/pubmed/id/11485197

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2001-8-3
pubmed:abstractText
The glutathione peroxidase homologous gene (Gpxh gene) in Chlamydomonas reinhardtii is up-regulated under oxidative stress conditions. The Gpxh gene showed a remarkably strong and fast induction by the singlet oxygen-generating photosensitizers neutral red, methylene blue and rose Bengal. The Gpxh mRNA levels strongly increased, albeit much more slowly, upon exposure to the organic hydroperoxides tert-butyl hydroperoxide (t-BOOH) and cumene hydroperoxide. In contrast, the Gpxh mRNA levels were only weakly induced by exposure to the superoxide-generating compound paraquat and by hydrogen peroxide. A comparison of the Gpxh mRNA levels with those of the heat shock protein HSP70A and the iron superoxide dismutase gene showed qualitative and quantitative differences for the three genes under oxidative stress conditions tested. The Gpxh gene is specifically induced by singlet-oxygen photosensitizers and the relative induction by other compounds is much weaker for Gpxh than for the other genes investigated. Using Gpxh promoter fusions with the arylsulfatase reporter gene, we have shown that the Gpxh was transcriptionally up-regulated by singlet-oxygen photosensitizers. It is also shown that the Gpxh promoter contains a region between 104 and 179 bp upstream of the transcription start that is responsible for the mRNA up-regulation upon exposure to 1O2 but not t-BOOH. Within this region a regulatory sequence homologous to the mammalian cAMP response element (CRE) and activator protein 1 (AP-1) binding site was identified within a 16 bp palindrome.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0167-4412
pubmed:author
pubmed:issnType
Print
pubmed:volume
46
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
395-408
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:11485197-Algal Proteins, pubmed-meshheading:11485197-Animals, pubmed-meshheading:11485197-Arylsulfatases, pubmed-meshheading:11485197-Chlamydomonas reinhardtii, pubmed-meshheading:11485197-Dose-Response Relationship, Drug, pubmed-meshheading:11485197-Glutathione Peroxidase, pubmed-meshheading:11485197-HSP70 Heat-Shock Proteins, pubmed-meshheading:11485197-Hydrogen Peroxide, pubmed-meshheading:11485197-Light, pubmed-meshheading:11485197-Oxidative Stress, pubmed-meshheading:11485197-Oxygen, pubmed-meshheading:11485197-Photosensitizing Agents, pubmed-meshheading:11485197-RNA, Messenger, pubmed-meshheading:11485197-Recombinant Fusion Proteins, pubmed-meshheading:11485197-Sequence Deletion, pubmed-meshheading:11485197-Singlet Oxygen, pubmed-meshheading:11485197-Superoxide Dismutase, pubmed-meshheading:11485197-Transcriptional Activation, pubmed-meshheading:11485197-Up-Regulation
pubmed:year
2001
pubmed:articleTitle
The glutathione peroxidase homologous gene from Chlamydomonas reinhardtii is transcriptionally up-regulated by singlet oxygen.
pubmed:affiliation
Department of Environmental Microbiology and Molecular Ecotoxicology, Swiss Federal Institute for Environmental Science and Technology (EAWAG), Duebendorf.
pubmed:publicationType
Journal Article