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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2001-8-3
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pubmed:abstractText |
It has been suggested that the activity of Ca2+/calmodulin-dependent protein kinase II (CaMKII) increases during acidosis in cardiac muscle. Thus we have investigated the role of CaMKII during acidosis by monitoring intracellular Ca2+ (using fura-2) and ICa (using the perforated patch clamp technique) during acidosis, in the absence and presence of the CaMKII inhibitor KN-93, in rat isolated ventricular myocytes. In the absence of KN-93, acidosis (pH 6.5) increased the amplitude of the fura-2 transient and prolonged its decay, but in the presence of KN-93 acidosis did not alter the amplitude and prolonged the decay more. In the absence of KN-93, acidosis increased the amplitude of the caffeine-induced fura-2 transient but did not alter its amplitude in the presence of KN-93. ICa did not change significantly during acidosis in the absence of KN-93 but decreased during acidosis in the presence of KN-93. These results suggest that activation of CaMKII during acidosis helps to compensate for the direct inhibitory effects of acidosis on sarcoplasmic reticular Ca2+ uptake and ICa.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Benzylamines,
http://linkedlifedata.com/resource/pubmed/chemical/Caffeine,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channels, L-Type,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Calmodulin-Dependent...,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Calmodulin-Dependent...,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Fura-2,
http://linkedlifedata.com/resource/pubmed/chemical/KN 93,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphodiesterase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Sulfonamides
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0031-6768
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
442
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
353-61
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:11484765-Acidosis,
pubmed-meshheading:11484765-Animals,
pubmed-meshheading:11484765-Benzylamines,
pubmed-meshheading:11484765-Caffeine,
pubmed-meshheading:11484765-Calcium,
pubmed-meshheading:11484765-Calcium Channels, L-Type,
pubmed-meshheading:11484765-Calcium-Calmodulin-Dependent Protein Kinase Type 2,
pubmed-meshheading:11484765-Calcium-Calmodulin-Dependent Protein Kinases,
pubmed-meshheading:11484765-Enzyme Inhibitors,
pubmed-meshheading:11484765-Fluorescent Dyes,
pubmed-meshheading:11484765-Fura-2,
pubmed-meshheading:11484765-Heart Ventricles,
pubmed-meshheading:11484765-Hydrogen-Ion Concentration,
pubmed-meshheading:11484765-Male,
pubmed-meshheading:11484765-Muscle Fibers, Skeletal,
pubmed-meshheading:11484765-Myocardium,
pubmed-meshheading:11484765-Patch-Clamp Techniques,
pubmed-meshheading:11484765-Phosphodiesterase Inhibitors,
pubmed-meshheading:11484765-Rats,
pubmed-meshheading:11484765-Rats, Wistar,
pubmed-meshheading:11484765-Sarcoplasmic Reticulum,
pubmed-meshheading:11484765-Sulfonamides
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pubmed:year |
2001
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pubmed:articleTitle |
Compensatory role of CaMKII on ICa and SR function during acidosis in rat ventricular myocytes.
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pubmed:affiliation |
School of Biomedical Sciences, University of Leeds, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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