|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
17
|
|
pubmed:dateCreated |
2001-8-2
|
|
pubmed:abstractText |
Intravenous infection of cats with feline immunodeficiency virus was used as a model system to study activation of virus replication in brain-resident microglial cells in vitro. Virus release by ramified microglial cells isolated from subclinically infected animals was detectable in cell-free tissue culture supernatant only by reverse transcription and nested PCR of gag-specific RNA sequences and not by virion-associated reverse transcriptase activity. In contrast, cocultivation of in vivo-infected microglial cells with mitogen-activated peripheral blood mononuclear cells (PBMC) regularly allows detection of high virus yields in cell-free tissue culture fluid. Besides uptake and multiplication of microglia-derived virus in PBMC, release of virus from microglia is stimulated by cell contact with PBMC. The data suggest that T lymphocytes patrolling the central nervous system could reactivate the semilatent state of lentiviruses in microglial cells in the course of clinically silent central nervous system infection.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11483754-10334385,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11483754-10400783,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11483754-10560658,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11483754-10600603,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11483754-10691542,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11483754-10704350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11483754-10718534,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11483754-10766246,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11483754-10871766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11483754-10871768,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11483754-10871771,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11483754-10878709,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11483754-10985245,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11483754-1310554,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11483754-1337399,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11483754-1513462,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11483754-1586136,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11483754-2112615,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11483754-2562941,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11483754-7485383,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11483754-7561697,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11483754-7745431,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11483754-8301319,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11483754-8337831,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11483754-8866743,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11483754-9382615,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11483754-9520995,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11483754-9658089,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11483754-9671961,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11483754-9811731,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11483754-9840291
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Sep
|
|
pubmed:issn |
0022-538X
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
75
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
8090-5
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:11483754-Animals,
pubmed-meshheading:11483754-Cats,
pubmed-meshheading:11483754-Cell Line,
pubmed-meshheading:11483754-Coculture Techniques,
pubmed-meshheading:11483754-Immunodeficiency Virus, Feline,
pubmed-meshheading:11483754-Lentivirus Infections,
pubmed-meshheading:11483754-Leukocytes, Mononuclear,
pubmed-meshheading:11483754-Lymphocyte Activation,
pubmed-meshheading:11483754-Microglia,
pubmed-meshheading:11483754-Virion,
pubmed-meshheading:11483754-Virus Activation,
pubmed-meshheading:11483754-Virus Replication
|
|
pubmed:year |
2001
|
|
pubmed:articleTitle |
In vitro activation of feline immunodeficiency virus in ramified microglial cells from asymptomatically infected cats.
|
|
pubmed:affiliation |
Institut für Medizinische Mikrobiologie und Hygiene, Universitätsklinikum Mannheim, Ruprecht-Karls-Universität Heidelberg, Mannheim, Germany. andreas.hein@imh.ma.uni-heidelberg.de
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
