Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2001-8-2
pubmed:abstractText
In this study, the in vitro immunomodulatory effect of concentrated juice of Citrus aurantifolia cv. swingle (Lime) was investigated. Clarified fresh lime juice was concentrated by freeze-drying. After dialysis against phosphate buffered saline and sterilization by a Millipore filter, it was used for further experiments. Immunogenic property of the CLJ extract was documented by production of specific polyclonal antibodies in rabbits. The immunomodulatory effect of the extract was tested in mitogen activated cultured mononuclear cells. The culture results indicated that proliferation of phytohemagglutinin (PHA) activated mononuclear cells were significantly inhibited by 250 and 500 microg/ml of CLJ extract, whereas only 500 microg/ml of the extract could inhibit proliferation of staphylococcal protein A (SPA) activated mononuclear cells (P<0.05). The abrogation of this inhibitory effect of the CLJ extract was noted by adding anti-CLJ antibody to the lymphocyte culture. Considering these data, it can be concluded that the CLJ extract possesses immunomodulatory principles, which may mainly be due to the protein components of the extract.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0378-8741
pubmed:author
pubmed:issnType
Print
pubmed:volume
77
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
85-90
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11483382-Adjuvants, Immunologic, pubmed-meshheading:11483382-Animals, pubmed-meshheading:11483382-Antibodies, pubmed-meshheading:11483382-Cell Division, pubmed-meshheading:11483382-Cells, Cultured, pubmed-meshheading:11483382-Citrus, pubmed-meshheading:11483382-Humans, pubmed-meshheading:11483382-Immunoblotting, pubmed-meshheading:11483382-Iran, pubmed-meshheading:11483382-Leukocytes, Mononuclear, pubmed-meshheading:11483382-Lymphocyte Activation, pubmed-meshheading:11483382-Lymphocytes, pubmed-meshheading:11483382-Mitogens, pubmed-meshheading:11483382-Phytohemagglutinins, pubmed-meshheading:11483382-Plant Extracts, pubmed-meshheading:11483382-Plant Lectins, pubmed-meshheading:11483382-Plant Proteins, pubmed-meshheading:11483382-Plants, Medicinal, pubmed-meshheading:11483382-Rabbits
pubmed:year
2001
pubmed:articleTitle
Immunomodulatory effect of concentrated lime juice extract on activated human mononuclear cells.
pubmed:affiliation
Immunology Department, Medical School, Shiraz University of Medical Sciences, PO Box 71345-1798, Shiraz, Iran.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't