Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
16
pubmed:dateCreated
2001-8-1
pubmed:abstractText
We report, to our knowledge, the first HIV type 1 (HIV-1) transgenic (Tg) rat. Expression of the transgene, consisting of an HIV-1 provirus with a functional deletion of gag and pol, is regulated by the viral long terminal repeat. Spliced and unspliced viral transcripts were expressed in lymph nodes, thymus, liver, kidney, and spleen, suggesting that Tat and Rev are functional. Viral proteins were identified in spleen tissue sections by immunohistochemistry and gp120 was present in splenic macrophages, T and B cells, and in serum. Clinical signs included wasting, mild to severe skin lesions, opaque cataracts, neurological signs, and respiratory difficulty. Histopathology included a selective loss of splenocytes within the periarterial lymphoid sheath, increased apoptosis of endothelial cells and splenocytes, follicular hyperplasia of the spleen, lymphocyte depletion of mesenteric lymph nodes, interstitial pneumonia, psoriatic skin lesions, and neurological, cardiac, and renal pathologies. Immunologically, delayed-type hypersensitivity response to keyhole limpet hemocyanin was diminished. By contrast, Ab titers and proliferative response to recall antigen (keyhole limpet hemocyanin) were normal. The HIV-1 Tg rat thus has many similarities to humans infected with HIV-1 in expression of viral genes, immune-response alterations, and pathologies resulting from infection. The HIV-1 Tg rat may provide a valuable model for some of the pathogenic manifestations of chronic HIV-1 diseases and could be useful in testing therapeutic regimens targeted to stages of viral replication subsequent to proviral integration.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/11481487-10201015, http://linkedlifedata.com/resource/pubmed/commentcorrection/11481487-10460507, http://linkedlifedata.com/resource/pubmed/commentcorrection/11481487-10469389, http://linkedlifedata.com/resource/pubmed/commentcorrection/11481487-10973276, http://linkedlifedata.com/resource/pubmed/commentcorrection/11481487-11024113, http://linkedlifedata.com/resource/pubmed/commentcorrection/11481487-1195397, http://linkedlifedata.com/resource/pubmed/commentcorrection/11481487-1690559, http://linkedlifedata.com/resource/pubmed/commentcorrection/11481487-1926769, http://linkedlifedata.com/resource/pubmed/commentcorrection/11481487-2195875, http://linkedlifedata.com/resource/pubmed/commentcorrection/11481487-2572240, http://linkedlifedata.com/resource/pubmed/commentcorrection/11481487-2609400, http://linkedlifedata.com/resource/pubmed/commentcorrection/11481487-2681968, http://linkedlifedata.com/resource/pubmed/commentcorrection/11481487-2845275, http://linkedlifedata.com/resource/pubmed/commentcorrection/11481487-3005516, http://linkedlifedata.com/resource/pubmed/commentcorrection/11481487-3072672, http://linkedlifedata.com/resource/pubmed/commentcorrection/11481487-7537511, http://linkedlifedata.com/resource/pubmed/commentcorrection/11481487-7909972, http://linkedlifedata.com/resource/pubmed/commentcorrection/11481487-8212579, http://linkedlifedata.com/resource/pubmed/commentcorrection/11481487-8506942, http://linkedlifedata.com/resource/pubmed/commentcorrection/11481487-8709786, http://linkedlifedata.com/resource/pubmed/commentcorrection/11481487-8855629, http://linkedlifedata.com/resource/pubmed/commentcorrection/11481487-8883175, http://linkedlifedata.com/resource/pubmed/commentcorrection/11481487-8941233, http://linkedlifedata.com/resource/pubmed/commentcorrection/11481487-9417563, http://linkedlifedata.com/resource/pubmed/commentcorrection/11481487-9491887, http://linkedlifedata.com/resource/pubmed/commentcorrection/11481487-9625388, http://linkedlifedata.com/resource/pubmed/commentcorrection/11481487-9718123, http://linkedlifedata.com/resource/pubmed/commentcorrection/11481487-9790524
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
31
pubmed:volume
98
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
9271-6
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
An HIV-1 transgenic rat that develops HIV-related pathology and immunologic dysfunction.
pubmed:affiliation
Animal Model Division and Divisions of Basic Science, University of Maryland, Baltimore, MD 21201, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't