Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
43
pubmed:dateCreated
2001-10-22
pubmed:abstractText
The activity of engineered, peptide-displaying enzymes is modulated by binding to specific anti-peptide antibodies. This new concept of a quantitative antibody detection system allows test kits to be set up for fast diagnosis of infectious diseases. To develop a quick and homogeneous assay for the detection of human immunodeficiency virus (HIV) infection, we have explored two acceptor sites of the bacterial Escherichia coli beta-galactosidase for the accommodation of HIV antigenic peptides. Two overlapping epitopes (namely P1 and P2) from the gp41 envelope glycoprotein, contained in different sized peptides, were inserted in the vicinity of the enzyme active site to generate a set of hybrid, enzymatically active beta-galactosidases. Regulable enzymes of different responsiveness to monoclonal antibody binding were generated with both acceptor sites tested. These biosensors were also sensitive to immune sera from HIV-infected patients. Modeling data provide insight into the structural modifications in the vicinity of the active site induced by peptide insertion that strongly affect the responsiveness of the engineered proteins through different parameters of their catalytic properties.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
26
pubmed:volume
276
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
40087-95
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:11479300-Amino Acid Sequence, pubmed-meshheading:11479300-Biosensing Techniques, pubmed-meshheading:11479300-Catalytic Domain, pubmed-meshheading:11479300-Enzyme Stability, pubmed-meshheading:11479300-Epitopes, pubmed-meshheading:11479300-Escherichia coli, pubmed-meshheading:11479300-HIV Antibodies, pubmed-meshheading:11479300-HIV Envelope Protein gp41, pubmed-meshheading:11479300-HIV Infections, pubmed-meshheading:11479300-HIV-1, pubmed-meshheading:11479300-Humans, pubmed-meshheading:11479300-Models, Molecular, pubmed-meshheading:11479300-Molecular Sequence Data, pubmed-meshheading:11479300-Peptide Fragments, pubmed-meshheading:11479300-Protein Engineering, pubmed-meshheading:11479300-Protein Structure, Quaternary, pubmed-meshheading:11479300-Protein Structure, Secondary, pubmed-meshheading:11479300-Recombinant Fusion Proteins, pubmed-meshheading:11479300-beta-Galactosidase
pubmed:year
2001
pubmed:articleTitle
Engineering regulable Escherichia coli beta-galactosidases as biosensors for anti-HIV antibody detection in human sera.
pubmed:affiliation
Institut de Biotecnologia i de Biomedicina and Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, 08193 Barcelona, Spain.
pubmed:publicationType
Journal Article, Clinical Trial, Comparative Study, Research Support, Non-U.S. Gov't