Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2001-7-31
pubmed:abstractText
A new micro-procedure for the quick regiospecific analysis of triacylglycerols (TAG) with a 1,3-specific lipase, Lipozyme IM 20 (produced from strains of Muccor miehei) is described. After dissolution of triacylglycerols in butan-1-ol or butan-2-ol 250 mg of Lipozyme IM 20 were added and the whole mixture was agitated. This operation was repeated 5-10 times and the esterified fatty acids in the form of butyl esters (FABE) were dissolved in pentane and washed with water. The pentane layer was dried over sodium sulfate and the solvent was removed under vacuum to a constant weight. The residue was analysed using GC-MS. The same triacylglycerols were converted to fatty acid methyl esters (FAME). The FAME were analysed by gas chromatography (GC).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0003-2654
pubmed:author
pubmed:issnType
Print
pubmed:volume
126
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1032-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
Quick regiospecific analysis of fatty acids in triacylglycerols with GC using 1,3-specific lipase in butanol.
pubmed:affiliation
Vioryl S.A., Kato Kifisia, Athens, Greece.
pubmed:publicationType
Journal Article