Source:http://linkedlifedata.com/resource/pubmed/id/11473927
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
2001-7-27
|
| pubmed:abstractText |
Female Asian elephants (Elephas maximus) release a pre-ovulatory urinary pheromone, (Z)-7-dodecenyl acetate (Z7--12:Ac), to signal males of their readiness to mate. Z7--12:Ac is quantitatively elevated during the follicular stage of estrus, reaching maximum concentrations just prior to ovulation, as demonstrated by two complementary headspace techniques: (i) evacuated canister capture followed by cryogenic trapping; (ii) solid phase microextraction (SPME), used prior to gas chromatography/ mass spectrometry (GC/MS). These patterns were coincident with observed male behaviors and were consistent with biochemical and binding properties of the active ligand, including optimal binding pH. To release maximum amounts of Z7--12:Ac for quantitation, serum and urine samples from three mature female Asian elephants in their luteal and follicular stages of several estrous cycles were subjected to heat and pH changes and were then treated with protease prior to SPME-GC/MS analyses. When the post-luteal serum progesterone concentrations declined to baseline levels, Z7--12:Ac became detectable in the female urine. Throughout the follicular stage pheromone concentrations increased linearly with no apparent relationship to the two serum luteinizing hormone peaks. Pre-ovulatory urine also contained related compounds, including (Z)-7--12-dodecenol. The relative amount of this alcohol increased relative to acetate during long-term storage, with a proportional reduction in bioactivity. Z7--12:Ac was not detected in mucus samples from the urogenital tract. A potential precursor of Z7--12:Ac was identified in liver homogenates from female elephants in the follicular stage.
|
| pubmed:grant | |
| pubmed:commentsCorrections | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acetates,
http://linkedlifedata.com/resource/pubmed/chemical/Fatty Acids, Monounsaturated,
http://linkedlifedata.com/resource/pubmed/chemical/Hormones,
http://linkedlifedata.com/resource/pubmed/chemical/Luteinizing Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/Pheromones
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0379-864X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
26
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
611-23
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:11473927-Acetates,
pubmed-meshheading:11473927-Age Factors,
pubmed-meshheading:11473927-Animals,
pubmed-meshheading:11473927-Behavior, Animal,
pubmed-meshheading:11473927-Elephants,
pubmed-meshheading:11473927-Fatty Acids, Monounsaturated,
pubmed-meshheading:11473927-Female,
pubmed-meshheading:11473927-Gas Chromatography-Mass Spectrometry,
pubmed-meshheading:11473927-Hormones,
pubmed-meshheading:11473927-Hot Temperature,
pubmed-meshheading:11473927-Hydrogen-Ion Concentration,
pubmed-meshheading:11473927-Liver,
pubmed-meshheading:11473927-Luteinizing Hormone,
pubmed-meshheading:11473927-Male,
pubmed-meshheading:11473927-Mass Spectrometry,
pubmed-meshheading:11473927-Ovulation,
pubmed-meshheading:11473927-Pheromones,
pubmed-meshheading:11473927-Protein Binding,
pubmed-meshheading:11473927-Time Factors
|
| pubmed:year |
2001
|
| pubmed:articleTitle |
Source and cyclic release pattern of (Z)-7-dodecenyl acetate, the pre-ovulatory pheromone of the female Asian elephant.
|
| pubmed:affiliation |
Department of Biochemistry and Molecular Biology, Oregon Graduate Institute of Science and Technology, Beaverton, OR 97006-8921, USA. bestr@bmb.ogi.edu
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|