Source:http://linkedlifedata.com/resource/pubmed/id/11472565
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
7
|
| pubmed:dateCreated |
2001-7-26
|
| pubmed:abstractText |
Gastrointestinal stromal tumor (GIST) is currently considered to be derived from the interstitial cells of Cajal (ICC). To test the hypothesis that omental mesenchymal tumor is also a type of GIST, we evaluated the expression of specific molecules in GIST, and c-kit gene mutation in omental mesenchymal tumors, and we identified a possible counterpart of ICC in the omentum. Immunohistochemically, all of the omental mesenchymal tumors (n = 5) were positive for both KIT and CD34, and three of the five tumors were also positive for an embryonic form of smooth-muscle myosin heavy chain (SMemb). Polymerase chain reaction-single-strand conformational polymorphism analysis (PCR-SSCP) and direct sequencing revealed mutations in c-kit gene exon 11 in all five tumors. As for the ICC counterparts in the omentum, there were some KIT-positive mesenchymal cells resembling ICC at the surface of the omentum. Double fluorescence immunostaining, using anti-KIT polyclonal antibodies and monoclonal antibodies against other molecules, demonstrated that KIT-, CD34- and SMemb-positive cells were present just beneath the mesothelial cells of the omentum. These results show that omental mesenchymal tumor corresponds to GIST of the omentum, and that KIT-positive bipolar mesenchymal cells may be a counterpart of ICC in the gastrointestinal tract. Identification of a new type of KIT-positive mesenchymal cell in the omentum may lead to the discovery of a new physiological role for this organ.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
1320-5463
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
51
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
524-31
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| pubmed:dateRevised |
2009-11-19
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| pubmed:meshHeading |
pubmed-meshheading:11472565-Adult,
pubmed-meshheading:11472565-Aged,
pubmed-meshheading:11472565-Amino Acid Sequence,
pubmed-meshheading:11472565-Base Sequence,
pubmed-meshheading:11472565-DNA, Neoplasm,
pubmed-meshheading:11472565-DNA Mutational Analysis,
pubmed-meshheading:11472565-DNA Primers,
pubmed-meshheading:11472565-Female,
pubmed-meshheading:11472565-Gastrointestinal Neoplasms,
pubmed-meshheading:11472565-Humans,
pubmed-meshheading:11472565-Immunoenzyme Techniques,
pubmed-meshheading:11472565-Middle Aged,
pubmed-meshheading:11472565-Molecular Sequence Data,
pubmed-meshheading:11472565-Myenteric Plexus,
pubmed-meshheading:11472565-Omentum,
pubmed-meshheading:11472565-Peritoneal Neoplasms,
pubmed-meshheading:11472565-Polymerase Chain Reaction,
pubmed-meshheading:11472565-Polymorphism, Single-Stranded Conformational,
pubmed-meshheading:11472565-Proto-Oncogene Proteins c-kit,
pubmed-meshheading:11472565-Stromal Cells,
pubmed-meshheading:11472565-Tumor Markers, Biological
|
| pubmed:year |
2001
|
| pubmed:articleTitle |
Gastrointestinal stromal tumors and KIT-positive mesenchymal cells in the omentum.
|
| pubmed:affiliation |
Department of Pathology, Jichi Medical School, Tochigi, Japan. ssakurai@jichi.ac.jp
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|