Source:http://linkedlifedata.com/resource/pubmed/id/11471795
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rdf:type | |
lifeskim:mentions |
umls-concept:C0001674,
umls-concept:C0001962,
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umls-concept:C0022702,
umls-concept:C0024488,
umls-concept:C0027950,
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umls-concept:C0033684,
umls-concept:C0183683,
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umls-concept:C0563532,
umls-concept:C1171411,
umls-concept:C1317973,
umls-concept:C1510827,
umls-concept:C1521721,
umls-concept:C1527178,
umls-concept:C1705938
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pubmed:issue |
2
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pubmed:dateCreated |
2001-7-26
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pubmed:abstractText |
A poly(vinyl alcohol)-based magnetic gel entrapping Fe3O4 colloids has been prepared by an emulsification-crosslinking method. The gel was modified with Cibacron blue 3GA, and thus a magnetic affinity support was produced. The adsorption equilibrium studies showed that the adsorption isotherm of lysozyme was nearly rectangular, with a capacity of 254 mg/ml, while the adsorption isotherm of bovine serum albumin obeyed the Henry's law. Uptake kinetics of the two proteins was investigated and analyzed with a pore diffusion model and a homogeneous diffusion model. Experimental results showed that the magnetic affinity gel had magnetic responsiveness and favorable properties in protein adsorption, and was mechanically and chemically stable.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0021-9673
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
6
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pubmed:volume |
921
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
109-19
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pubmed:dateRevised |
2009-1-15
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pubmed:meshHeading | |
pubmed:year |
2001
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pubmed:articleTitle |
Protein adsorption equilibria and kinetics to a poly(vinyl alcohol)-based magnetic affinity support.
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pubmed:affiliation |
Department of Biochemical Engineering, Tianjin University, China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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