Source:http://linkedlifedata.com/resource/pubmed/id/11468408
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
Pt 8
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pubmed:dateCreated |
2001-7-24
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pubmed:abstractText |
The small GTPase ARL2 (from Mus musculus) and an effector protein, the delta subunit of human cGMP phosphodiesterase (hPDE delta), were coexpressed and copurified from Escherichia coli as a stable complex. Coexpression significantly increased the otherwise low yield of PDE delta production in E. coli. The complex, which contains ARL2 in the activated GTP-bound form, was crystallized in two forms. The first belongs to the monoclinic space group P2(1), with unit-cell parameters a = 48.1, b = 45.7, c = 74.7 A, beta = 94.0 degrees and one complex (39 kDa) in the asymmetric unit. Cryocooled crystals diffract to 2.3 A using synchrotron radiation. The micro-focused X-ray beam at beamline ID13 (ESRF) allowed the use of very small crystals, which helped to overcome twinning and enabled the identification of a molecular-replacement solution. The second form recrystallized from the first one after several months. These crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 44.5, b = 65.4, c = 104.4 A and one complex in the asymmetric unit. They diffracted to 1.8 A using synchrotron radiation.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/3',5'-Cyclic-GMP Phosphodiesterases,
http://linkedlifedata.com/resource/pubmed/chemical/ARL2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic Nucleotide...,
http://linkedlifedata.com/resource/pubmed/chemical/Eye Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Guanosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/PDE6B protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Pde6b protein, mouse
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0907-4449
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
57
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1167-70
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:11468408-3',5'-Cyclic-GMP Phosphodiesterases,
pubmed-meshheading:11468408-Animals,
pubmed-meshheading:11468408-Crystallization,
pubmed-meshheading:11468408-Crystallography, X-Ray,
pubmed-meshheading:11468408-Cyclic Nucleotide Phosphodiesterases, Type 6,
pubmed-meshheading:11468408-Escherichia coli,
pubmed-meshheading:11468408-Eye Proteins,
pubmed-meshheading:11468408-GTP-Binding Proteins,
pubmed-meshheading:11468408-Guanosine Triphosphate,
pubmed-meshheading:11468408-Humans,
pubmed-meshheading:11468408-Mice,
pubmed-meshheading:11468408-Protein Conformation
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pubmed:year |
2001
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pubmed:articleTitle |
Coexpression, copurification, crystallization and preliminary X-ray analysis of a complex of ARL2-GTP and PDE delta.
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pubmed:affiliation |
Max-Planck-Institut für Molekulare Physiologie, Abteilung Strukturelle Biologie, Otto-Hahn-Strasse 11, 44227 Dortmund, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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