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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
16
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pubmed:dateCreated |
2001-7-23
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pubmed:abstractText |
We previously described a bacteriophage of the Lyme disease agent Borrelia burgdorferi designated phiBB-1. This phage packages the host complement of the 32-kb circular plasmids (cp32s), a group of homologous molecules found throughout the genus Borrelia. To demonstrate the ability of phiBB-1 to package and transduce DNA, a kanamycin resistance cassette was inserted into a cloned fragment of phage DNA, and the resulting construct was transformed into B. burgdorferi CA-11.2A cells. The kan cassette recombined into a resident cp32 and was stably maintained. The cp32 containing the kan cassette was packaged by phiBB-1 released from this B. burgdorferi strain. phiBB-1 has been used to transduce this antibiotic resistance marker into naive CA-11.2A cells, as well as two other strains of B. burgdorferi. This is the first direct evidence of a mechanism for lateral gene transfer in B. burgdorferi.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11466280-10024606,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11466280-10531261,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11466280-10572135,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/11466280-10678944,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/11466280-11004167,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/11466280-11106398,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11466280-11169111,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11466280-1905257,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/11466280-6828118,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11466280-685100,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/11466280-9695920
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
183
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4771-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:11466280-Bacteriophages,
pubmed-meshheading:11466280-Borrelia burgdorferi Group,
pubmed-meshheading:11466280-Cloning, Molecular,
pubmed-meshheading:11466280-DNA, Bacterial,
pubmed-meshheading:11466280-DNA, Viral,
pubmed-meshheading:11466280-DNA Transposable Elements,
pubmed-meshheading:11466280-Genetic Complementation Test,
pubmed-meshheading:11466280-Kanamycin Resistance,
pubmed-meshheading:11466280-Plasmids,
pubmed-meshheading:11466280-Polymerase Chain Reaction,
pubmed-meshheading:11466280-Restriction Mapping,
pubmed-meshheading:11466280-Transduction, Genetic,
pubmed-meshheading:11466280-Transformation, Genetic
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pubmed:year |
2001
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pubmed:articleTitle |
Transduction by phiBB-1, a bacteriophage of Borrelia burgdorferi.
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pubmed:affiliation |
Division of Biological Sciences, The University of Montana, Missoula, Montana 59812, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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