Source:http://linkedlifedata.com/resource/pubmed/id/11463496
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2001-7-20
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| pubmed:abstractText |
The effect of CD44-phenotypic expression on metastasis to the lung was studied using a spontaneous murine osteosarcoma-derived cell line, POS-1, stimulated with lipopolysaccharide (LPS). POS-1 cells were inoculated into the hind paws of 20 C3H/HeJ mice and produced a visible mass in all mice in 5 weeks, and these transplanted tumors resulted in lung metastasis in all mice. The number of metastatic foci in the lungs was 12.0+/-2.1 (mean+/-SD) with LPS-stimulated cells, which was significantly higher than that of unstimulated cells (5.8+/-1.4; N=10 for each; P<0.05). Hyaluronate (HA), a ligand of CD44, inhibited a number of lung metastases in a dose-dependent manner (0.5% HA, 3.0+/-1.1; 0.005% HA, 5.1+/-1.5; without HA, 8.6+/-1.7; N=10 for each; P<0.05, each group with HA versus the group without HA). Adhesion assay by coculturing POS-1 cells and lung microvascular endothelial cells on culture plate showed that the adhesion was significantly lower in HA treated POS-1 than those without HA (1.18+/-0.12 and 2.74+/-0.17, respectively, P<0.05). These results suggest that lung metastasis was accelerated by up-regulation of CD44.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Sep
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| pubmed:issn |
0304-3835
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
20
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| pubmed:volume |
170
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
177-82
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| pubmed:dateRevised |
2003-11-14
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| pubmed:meshHeading |
pubmed-meshheading:11463496-Animals,
pubmed-meshheading:11463496-Antigens, CD44,
pubmed-meshheading:11463496-Cell Adhesion,
pubmed-meshheading:11463496-Disease Models, Animal,
pubmed-meshheading:11463496-Endothelium, Vascular,
pubmed-meshheading:11463496-Lipopolysaccharides,
pubmed-meshheading:11463496-Lung Neoplasms,
pubmed-meshheading:11463496-Mice,
pubmed-meshheading:11463496-Mice, Inbred C3H,
pubmed-meshheading:11463496-Neoplasm Invasiveness,
pubmed-meshheading:11463496-Neoplasm Transplantation,
pubmed-meshheading:11463496-Osteosarcoma,
pubmed-meshheading:11463496-Tumor Cells, Cultured,
pubmed-meshheading:11463496-Up-Regulation
|
| pubmed:year |
2001
|
| pubmed:articleTitle |
Acceleration of lung metastasis by up-regulation of CD44 expression in osteosarcoma-derived cell transplanted mice.
|
| pubmed:affiliation |
Department of Orthopaedic Surgery, Yokohama City University School of Medicine, 3-9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan. hiro-s@qc4.so-net.ne.jp
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| pubmed:publicationType |
Journal Article
|