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rdf:type |
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lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0017337,
umls-concept:C0162610,
umls-concept:C0185117,
umls-concept:C0439849,
umls-concept:C0441712,
umls-concept:C0445223,
umls-concept:C0851285,
umls-concept:C1136031,
umls-concept:C1136087,
umls-concept:C1552599,
umls-concept:C1704787,
umls-concept:C1752426,
umls-concept:C2587213,
umls-concept:C2911684
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|
pubmed:issue |
1
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pubmed:dateCreated |
2001-7-19
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|
pubmed:abstractText |
RNAi is a gene-silencing phenomenon triggered by double-stranded (ds) RNA and involves the generation of 21 to 26 nt RNA segments that guide mRNA destruction. In Caenorhabditis elegans, lin-4 and let-7 encode small temporal RNAs (stRNAs) of 22 nt that regulate stage-specific development. Here we show that inactivation of genes related to RNAi pathway genes, a homolog of Drosophila Dicer (dcr-1), and two homologs of rde-1 (alg-1 and alg-2), cause heterochronic phenotypes similar to lin-4 and let-7 mutations. Further we show that dcr-1, alg-1, and alg-2 are necessary for the maturation and activity of the lin-4 and let-7 stRNAs. Our findings suggest that a common processing machinery generates guide RNAs that mediate both RNAi and endogenous gene regulation.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0092-8674
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
13
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pubmed:volume |
106
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
23-34
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:11461699-Animals,
pubmed-meshheading:11461699-Animals, Genetically Modified,
pubmed-meshheading:11461699-Caenorhabditis elegans,
pubmed-meshheading:11461699-DNA Primers,
pubmed-meshheading:11461699-Drosophila,
pubmed-meshheading:11461699-Embryo, Nonmammalian,
pubmed-meshheading:11461699-Female,
pubmed-meshheading:11461699-Gene Expression Regulation, Developmental,
pubmed-meshheading:11461699-Gene Silencing,
pubmed-meshheading:11461699-Genes, Helminth,
pubmed-meshheading:11461699-Genes, Reporter,
pubmed-meshheading:11461699-Genomic Imprinting,
pubmed-meshheading:11461699-Heterozygote,
pubmed-meshheading:11461699-Larva,
pubmed-meshheading:11461699-Luciferases,
pubmed-meshheading:11461699-Phylogeny,
pubmed-meshheading:11461699-Polymerase Chain Reaction,
pubmed-meshheading:11461699-RNA, Helminth
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pubmed:year |
2001
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pubmed:articleTitle |
Genes and mechanisms related to RNA interference regulate expression of the small temporal RNAs that control C. elegans developmental timing.
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pubmed:affiliation |
Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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