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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2001-7-18
pubmed:abstractText
Coregulators have been suggested to act as a bridging apparatus between nuclear receptors and the transcriptional machinery. The orphan receptor SF-1 plays a role in controlling the basal and cAMP-stimulated expression of the human steroidogenic acute regulatory protein gene. DAX-1 is the gene responsible for X-linked adrenal hypoplasia congenita and blocks steroid biosynthesis by impairing the expression of steroidogenic acute regulatory protein. In the present study we examined the role of coregulators in the actions of SF-1 and DAX-1 on the human steroidogenic acute regulatory protein promoter. We found that the coregulator RIP 140 interacts with SF-1 in the yeast two-hybrid system. Glutathione-S-transferase pull-down assays and coimmunoprecipitations confirmed the interaction between RIP 140 and SF-1. RIP 140 was also shown to interact with DAX-1. When an RIP 140 expression vector was introduced into Y-1 cells, basal and cAMP-stimulated human steroidogenic acute regulatory protein promoter activities decreased. The inhibitory effect of RIP 140 on human steroidogenic acute regulatory protein promoter activity was dependent upon the presence of SF-1. The cAMP response of an SF-1 response element was inhibited by both RIP 140 and DAX-1 expression vectors at low concentrations of plasmids. We conclude that RIP 140 binds to the orphan nuclear receptor SF-1 and DAX-1 and modulates their actions on the human steroidogenic acute regulatory protein promoter.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Signal Transducing, http://linkedlifedata.com/resource/pubmed/chemical/DAX-1 Orphan Nuclear Receptor, http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Fushi Tarazu Transcription Factors, http://linkedlifedata.com/resource/pubmed/chemical/Homeodomain Proteins, http://linkedlifedata.com/resource/pubmed/chemical/NR0B1 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Nr0b1 protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cytoplasmic and Nuclear, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Retinoic Acid, http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Steroidogenic Factor 1, http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors, http://linkedlifedata.com/resource/pubmed/chemical/nuclear receptor interacting..., http://linkedlifedata.com/resource/pubmed/chemical/steroidogenic acute regulatory...
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0013-7227
pubmed:author
pubmed:issnType
Print
pubmed:volume
142
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3570-7
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:11459805-Adaptor Proteins, Signal Transducing, pubmed-meshheading:11459805-Animals, pubmed-meshheading:11459805-COS Cells, pubmed-meshheading:11459805-Cricetinae, pubmed-meshheading:11459805-DAX-1 Orphan Nuclear Receptor, pubmed-meshheading:11459805-DNA-Binding Proteins, pubmed-meshheading:11459805-Fushi Tarazu Transcription Factors, pubmed-meshheading:11459805-Homeodomain Proteins, pubmed-meshheading:11459805-Humans, pubmed-meshheading:11459805-Mice, pubmed-meshheading:11459805-Nuclear Proteins, pubmed-meshheading:11459805-Phosphoproteins, pubmed-meshheading:11459805-Promoter Regions, Genetic, pubmed-meshheading:11459805-Receptors, Cytoplasmic and Nuclear, pubmed-meshheading:11459805-Receptors, Retinoic Acid, pubmed-meshheading:11459805-Repressor Proteins, pubmed-meshheading:11459805-Steroidogenic Factor 1, pubmed-meshheading:11459805-Transcription, Genetic, pubmed-meshheading:11459805-Transcription Factors, pubmed-meshheading:11459805-Tumor Cells, Cultured
pubmed:year
2001
pubmed:articleTitle
RIP 140 modulates transcription of the steroidogenic acute regulatory protein gene through interactions with both SF-1 and DAX-1.
pubmed:affiliation
Department of Biochemistry, Hokkaido University School of Medicine, Sapporo, Hokkaido 060-8638, Japan. terusuga@med.hokudai.ac.jp
pubmed:publicationType
Journal Article