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| pubmed-article:11457968 | lifeskim:mentions | umls-concept:C0016126 | lld:lifeskim |
| pubmed-article:11457968 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:11457968 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
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| pubmed-article:11457968 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:11457968 | pubmed:dateCreated | 2001-7-17 | lld:pubmed |
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| pubmed-article:11457968 | pubmed:abstractText | The cloning of two highly homologous chicory (Cichorium intybus var. foliosum cv Flash) fructan 1-exohydrolase cDNAs (1-FEH IIa and 1-FEH IIb) is described. Both isoenzymes could be purified from forced chicory roots as well as from the etiolated "Belgian endive" leaves where the 1-FEH IIa isoform is present in higher concentrations. Full-length cDNAs were obtained by a combination of reverse transcriptase-polymerase chain reaction (PCR), PCR and 5'- and 3'-rapid amplification of cDNA ends using primers based on N-terminal and conserved amino acid sequences. 1-FEH IIa and 1-FEH IIb cDNA-derived amino acid sequences are most homologous to a new group of plant glycosyl hydrolases harboring cell wall-type enzymes with acid isoelectric points. Unlike the observed expression profiles of chicory 1-FEH I, northern analysis revealed that 1-FEH II is expressed when young chicory plants are defoliated, suggesting that this enzyme can be induced at any developmental stage when large energy supplies are necessary (regrowth after defoliation). | lld:pubmed |
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| pubmed-article:11457968 | pubmed:language | eng | lld:pubmed |
| pubmed-article:11457968 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:11457968 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:11457968 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:11457968 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:11457968 | pubmed:issn | 0032-0889 | lld:pubmed |
| pubmed-article:11457968 | pubmed:author | pubmed-author:Van LaereA... | lld:pubmed |
| pubmed-article:11457968 | pubmed:author | pubmed-author:De RooverJJ | lld:pubmed |
| pubmed-article:11457968 | pubmed:author | pubmed-author:Van den... | lld:pubmed |
| pubmed-article:11457968 | pubmed:author | pubmed-author:MichielsAA | lld:pubmed |
| pubmed-article:11457968 | pubmed:author | pubmed-author:Van... | lld:pubmed |
| pubmed-article:11457968 | pubmed:author | pubmed-author:ClerensS PSP | lld:pubmed |
| pubmed-article:11457968 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:11457968 | pubmed:volume | 126 | lld:pubmed |
| pubmed-article:11457968 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:11457968 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:11457968 | pubmed:pagination | 1186-95 | lld:pubmed |
| pubmed-article:11457968 | pubmed:dateRevised | 2010-9-14 | lld:pubmed |
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| pubmed-article:11457968 | pubmed:year | 2001 | lld:pubmed |
| pubmed-article:11457968 | pubmed:articleTitle | Defoliation induces fructan 1-exohydrolase II in Witloof chicory roots. Cloning and purification of two isoforms, fructan 1-exohydrolase IIa and fructan 1-exohydrolase IIb. Mass fingerprint of the fructan 1-exohydrolase II enzymes. | lld:pubmed |
| pubmed-article:11457968 | pubmed:affiliation | Department of Biology, Laboratory for Developmental Biology, Botany Institute, K.U. Leuven, Kasteelpark Arenberg 31, B-3001 Leuven, Belgium. wim.vandenende@bio.kuleuven.ac.be | lld:pubmed |
| pubmed-article:11457968 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:11457968 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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