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rdf:type |
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lifeskim:mentions |
umls-concept:C0009015,
umls-concept:C0014442,
umls-concept:C0016126,
umls-concept:C0205263,
umls-concept:C0242726,
umls-concept:C0577559,
umls-concept:C0597298,
umls-concept:C0969434,
umls-concept:C0969435,
umls-concept:C0969438,
umls-concept:C1145671,
umls-concept:C1998793
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pubmed:issue |
3
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pubmed:dateCreated |
2001-7-17
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pubmed:databankReference |
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pubmed:abstractText |
The cloning of two highly homologous chicory (Cichorium intybus var. foliosum cv Flash) fructan 1-exohydrolase cDNAs (1-FEH IIa and 1-FEH IIb) is described. Both isoenzymes could be purified from forced chicory roots as well as from the etiolated "Belgian endive" leaves where the 1-FEH IIa isoform is present in higher concentrations. Full-length cDNAs were obtained by a combination of reverse transcriptase-polymerase chain reaction (PCR), PCR and 5'- and 3'-rapid amplification of cDNA ends using primers based on N-terminal and conserved amino acid sequences. 1-FEH IIa and 1-FEH IIb cDNA-derived amino acid sequences are most homologous to a new group of plant glycosyl hydrolases harboring cell wall-type enzymes with acid isoelectric points. Unlike the observed expression profiles of chicory 1-FEH I, northern analysis revealed that 1-FEH II is expressed when young chicory plants are defoliated, suggesting that this enzyme can be induced at any developmental stage when large energy supplies are necessary (regrowth after defoliation).
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/11457968-10028694,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11457968-10209134,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11457968-10217779,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11457968-10364386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11457968-10482654,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11457968-10632723,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11457968-10652142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11457968-10713449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11457968-10805453,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11457968-10806226,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11457968-10937702,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11457968-11115126,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11457968-1783180,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11457968-6329026,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11457968-7847635,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11457968-8016265,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11457968-8742337,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11457968-9306701,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11457968-9430596,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11457968-9829697,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11457968-9891456,
http://linkedlifedata.com/resource/pubmed/commentcorrection/11457968-9951726
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0032-0889
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
126
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1186-95
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pubmed:dateRevised |
2010-9-14
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pubmed:meshHeading |
pubmed-meshheading:11457968-Amino Acid Sequence,
pubmed-meshheading:11457968-Chicory,
pubmed-meshheading:11457968-Cloning, Molecular,
pubmed-meshheading:11457968-Exons,
pubmed-meshheading:11457968-Glycoside Hydrolases,
pubmed-meshheading:11457968-Isoenzymes,
pubmed-meshheading:11457968-Molecular Sequence Data,
pubmed-meshheading:11457968-Peptide Fragments,
pubmed-meshheading:11457968-Plant Leaves,
pubmed-meshheading:11457968-Plant Roots,
pubmed-meshheading:11457968-Sequence Homology, Amino Acid,
pubmed-meshheading:11457968-beta-Fructofuranosidase
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pubmed:year |
2001
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pubmed:articleTitle |
Defoliation induces fructan 1-exohydrolase II in Witloof chicory roots. Cloning and purification of two isoforms, fructan 1-exohydrolase IIa and fructan 1-exohydrolase IIb. Mass fingerprint of the fructan 1-exohydrolase II enzymes.
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pubmed:affiliation |
Department of Biology, Laboratory for Developmental Biology, Botany Institute, K.U. Leuven, Kasteelpark Arenberg 31, B-3001 Leuven, Belgium. wim.vandenende@bio.kuleuven.ac.be
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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