Linked Life Data

11455426

Source:http://linkedlifedata.com/resource/pubmed/id/11455426

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2001-7-16
pubmed:databankReference
pubmed:abstractText
We examined the spatial expression pattern of an orphan receptor guanylyl cyclase gene, OlGC3, during embryogenesis of medaka, and we analyzed the cis-regulatory region required for its photoreceptor cell-specific expression by introducing promoter-green fluorescent protein gene (GFP) fusion constructs into medaka embryos. Zygotic expression of OlGC3 first appears at stage 36 and is confined to retinal photoreceptor cells. The 5' flanking sequence up to -388 is sufficient to drive the photoreceptor cell-specific gene expression. When a mutation was introduced into the orthodenticle-related homeobox (OTX)-binding consensus sequence at -36/-31, reporter gene expression was completely abolished in retinal photoreceptor cells. The mutation seemed not to impair promoter elements for general transcription factors, because ectopic GFP expression in yolk epithelium was not diminished by the same mutation. These results suggest that the proximal cis-regulatory element containing a consensus binding sequence for OTX transcription factors is essential for OlGC3 expression in photoreceptor cells.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0949-944X
pubmed:author
pubmed:issnType
Print
pubmed:volume
211
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
145-9
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
A cis-regulatory element essential for photoreceptor cell-specific expression of a medaka retinal guanylyl cyclase gene.
pubmed:affiliation
Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo 060-0810, Japan. tgk@sci.himeji-tech.ac.jp
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't